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Browse MedicagoPLEX Experiment Data

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Gene expression data from Medicago truncatula plantlet roots treated with symbiotic lipochitooligosaccharides (LCOs).
Helge Kuster, Leibniz Universität Hannover, Institut für Pflanzengenetik, Hannover, Germany (helge.kuester@genetik.uni-hannover.de)
Experiment design (36 hybridizations)
compound
•sMyc-LCOs •nsMyc-LCOs •s/nsMyc-LCOs •Myc-LCOs-control •Nod-LCOs •Nod-LCO-control
time
•6 hrs •24 hrs

This experiment has been imported by PLEXdb from NCBI GEO (GSE33636)

Series_summary:
Legumes interact with soil microbes, leading to ...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Gene expression data from Medicago truncatula plantlet roots treated with symbiotic lipochitooligosaccharides (LCOs).
Accession No: ME22
Microarray: Medicago61k
Visibility: public
Experiment Type:
Experiment Factor(s):
compound
•sMyc-LCOs   •nsMyc-LCOs   •s/nsMyc-LCOs   •Myc-LCOs-control   •Nod-LCOs   •Nod-LCO-control
time
•6 hrs  •24 hrs
Quality Control: biological replicates
Treatment summary:
 compound  time  # replicates
 sMyc-LCOs  6 hrs  3
 sMyc-LCOs  24 hrs  3
 nsMyc-LCOs  6 hrs  3
 nsMyc-LCOs  24 hrs  3
 s/nsMyc-LCOs  6 hrs  3
 s/nsMyc-LCOs  24 hrs  3
 Myc-LCOs-control  6 hrs  3
 Myc-LCOs-control  24 hrs  3
 Nod-LCOs  6 hrs  3
 Nod-LCOs  24 hrs  3
 Nod-LCO-control  6 hrs  3
 Nod-LCO-control  24 hrs  3
Total hybridizations: 36
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE33636)

Series_summary:
Legumes interact with soil microbes, leading to the development of nitrogen-fixing root nodules and arbuscular mycorrhizal (AM) roots. While nodule initiation by diffusible lipochitooligosaccharide (LCO) Nod-factors of bacterial origin (Nod-LCOs) is well characterized, diffusible AM fungal signals were only recently identified as sulphated and non-sulphated LCOs (sMyc-LCOs and nsMyc-LCOs).
Applying Myc-LCOs in parallel to Nod-LCOs, we used GeneChips to detail the global programme of gene expression in response to the external application of symbiotic LCOs.

Series_overall_design:
To harvest tissues for transcriptome profiling, three biological replicates consisting of 20 plantlets per treatment were selected. After 6 h of incubation in the climate chamber, 10 plantlets per batch were removed from the treatment (Myc-LCOs or Nod-LCOs) or control solutions and harvested, while the other 10 remained in the respective solutions for a total of 24 h. During harvest, one mm of the root tip of each plantlet was removed and discarded. The remaining 2 to 2.5 cm of the distal root region were cut off and directly frozen in liquid nitrogen.


PLEXdb Curator's Note:
Subseries *GSE33636 (ME22) and GSE33637(ME23) are part of superseries GSE33638 (ME24) at GEO.
Publication: 'Transcriptional responses towards diffusible signals from symbiotic microbes reveal MtNFP- and MtDMI3-dependent reprogramming of host gene expression by AM fungal LCOs.', Czaja LF, Hogekamp C, Lamm P, Maillet F, Andres Martinez E, Samain E, Dénarié J, Küster H, Hohnjec N.
Plant Physiol 2012 May 31.
pubmed: 22652128
Created: 2012-06-28 11:04:33
Last Update: 2012-06-28 15:11:01
Released: 2012-07-03
GEO Accession GSE33636
Submitter: PLEXdb Curator
Name: Helge Kuster
Institution: Leibniz Universität Hannover, Institut für Pflanzengenetik, Hannover, Germany
Head of Laboratory: Helge Kuster
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