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Browse MedicagoPLEX Experiment Data

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Gene expression data from Medicago truncatula mutant plantlet roots treated with Myc-LCOs.
Helge Kuster, Leibniz Universität Hannover, Institut für Pflanzengenetik, Hannover, Germany (helge.kuester@genetik.uni-hannover.de)
Experiment design (24 hybridizations)
compound
•sMyc-LCOs •nsMyc-LCOs •s/nsMyc-LCOs •control
genotype
•NFP mutant •DM13 mutant

This experiment has been imported by PLEXdb from NCBI GEO (GSE33637)

Series_summary:
Legumes interact with soil fungi, leading to the...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Gene expression data from Medicago truncatula mutant plantlet roots treated with Myc-LCOs.
Accession No: ME23
Microarray: Medicago61k
Visibility: public
Experiment Type:
Experiment Factor(s):
compound
•sMyc-LCOs   •nsMyc-LCOs   •s/nsMyc-LCOs   •control
genotype
•NFP mutant   •DM13 mutant
Quality Control: biological replicates
Treatment summary:
 compound  genotype  # replicates
 sMyc-LCOs  NFP mutant  3
 sMyc-LCOs  DM13 mutant  3
 nsMyc-LCOs  NFP mutant  3
 nsMyc-LCOs  DM13 mutant  3
 s/nsMyc-LCOs  NFP mutant  3
 s/nsMyc-LCOs  DM13 mutant  3
 control  NFP mutant  3
 control  DM13 mutant  3
Total hybridizations: 24
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE33637)

Series_summary:
Legumes interact with soil fungi, leading to the development of arbuscular mycorrhizal (AM) roots. Diffusible AM fungal signals were identified as sulphated and non-sulphated LCOs (sMyc-LCOs and nsMyc-LCOs).
Applying Myc-LCOs on roots of symbiotic mutants, we used GeneChips to detail the global programme of gene expression in these mutants in response to the external application of Myc-LCOs.

Series_overall_design:
To harvest tissues for transcriptome profiling, three biological replicates consisting of 10 plantlets per treatment were selected. After 6 h of incubation in the climate chamber, 10 plantlets per batch were removed from the treatment (Myc-LCOs) or control solutions and harvested. During harvest, one mm of the root tip of each plantlet was removed and discarded. The remaining 2 to 2.5 cm of the distal root region were cut off and directly frozen in liquid nitrogen.


PLEXdb Curator's Note:
Subseries GSE33636 (ME22) and *GSE33637(ME23) are part of superseries GSE33638 (ME24) at GEO.
Publication: 'Transcriptional responses towards diffusible signals from symbiotic microbes reveal MtNFP- and MtDMI3-dependent reprogramming of host gene expression by AM fungal LCOs.', Czaja LF, Hogekamp C, Lamm P, Maillet F, Andres Martinez E, Samain E, Dénarié J, Küster H, Hohnjec N.
Plant Physiol 2012 May 31.
pubmed: 22652128
Created: 2012-06-28 12:07:51
Last Update: 2012-06-28 14:39:02
Released: 2012-07-03
GEO Accession GSE33637
Submitter: PLEXdb Curator
Name: Helge Kuster
Institution: Leibniz Universität Hannover, Institut für Pflanzengenetik, Hannover, Germany
Head of Laboratory: Helge Kuster
email(s):
Homepage:

 
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