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Gene expression data from phosphinothricin (PPT) treated Medicago truncatula root nodules
Helena Carvalho, Instituto de Biologia Molecular e Celular, Department of MBNA, Porto, Portugal (mhcarval@ibmc.up.pt)
Experiment design (12 hybridizations)
compound
•0 hr •4 hr •8 hr •24 hr

This experiment has been imported by PLEXdb from NCBI GEO (GSE34155)

Series_summary:
Nitrogen assimilation in plants is a tightly reg...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Gene expression data from phosphinothricin (PPT) treated Medicago truncatula root nodules
Accession No: ME25
Microarray: Medicago61k
Visibility: public
Experiment Type:
Experiment Factor(s):
compound
•0 hr   •4 hr   •8 hr   •24 hr
Quality Control: biological replicates
Treatment summary:
 compound  # replicates
 0 hr  3
 4 hr  3
 8 hr  3
 24 hr  3
Total hybridizations: 12
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE34155)

Series_summary:
Nitrogen assimilation in plants is a tightly regulated process that integrates developmental and environmental signals. The legume-rhizobial symbiosis results in the formation of a specialized organ called root nodule, where the rhizobia fixes atmospheric nitrogen into ammonia. Ammonia is assimilated by the plant enzyme glutamine synthetase, which is specifically inhibited by PPT. The expression of key genes related to the regulation of root nodule metabolism will likely be affected by glutamine synthetase inhibition.
We used microarrays to detail the global programme of gene expression in response to Glutamine synthetase inhibition in root nodules and identified genes differentially expressed over a time course.

Series_overall_design:
Medicago truncatula nodulated plants (20 days post inoculation) were treated with 0.25 mM of PPT. Root nodules were harvested at 4, 8 and 24 hours after PPT application. As a control, root nodules collected just before PPT application were used (PPT 0h). Three biological replicates consisting of pools of root nodules harvested from five distinct plants were used for RNA extraction and hybridization on Affymetrix GeneChips.
Publication: 'Inhibition of Glutamine Synthetase by Phosphinothricin Leads to Transcriptome Reprograming in Root Nodules of Medicago truncatula.', Seabra AR, Pereira PA, Becker JD, Carvalho HG.
Mol Plant Microbe Interact 2012 Jul;25(7):976-92.
pubmed: 22414438
Created: 2012-07-03 10:54:34
Last Update: 2012-07-03 13:57:22
Released: 2012-07-10
GEO Accession GSE34155
Submitter: PLEXdb Curator
Name: Helena Carvalho
Institution: Instituto de Biologia Molecular e Celular, Department of MBNA, Porto, Portugal
Head of Laboratory: Helena Carvalho
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