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Browse MedicagoPLEX Experiment Data

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affy_med_2011_06-Analysis of the Medicago noot mutant
Stephanie Huguet, INRA, Evry, France (huguet@evry.inra.fr)
Experiment design (10 hybridizations)
inoculation
•WT root uninoculated •WT nodules inoculated •noot NF2717 nodules inoculated •noot Tnk507 nodules inoculated

This experiment has been imported by PLEXdb from NCBI GEO (GSE36558)

Series_summary:
affy_med_2011_06 - affy_med_2011_06 - Legume pla...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: affy_med_2011_06-Analysis of the Medicago noot mutant
Accession No: ME27
Microarray: Medicago61k
Visibility: public
Experiment Type:
Experiment Factor(s):
inoculation
•WT root uninoculated   •WT nodules inoculated   •noot NF2717 nodules inoculated   •noot Tnk507 nodules inoculated
Quality Control: biological replicates
Treatment summary:
 inoculation  # replicates
 WT root uninoculated  3
 WT nodules inoculated  3
 noot NF2717 nodules inoculated  2
 noot Tnk507 nodules inoculated  2
Total hybridizations: 10
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE36558)

Series_summary:
affy_med_2011_06 - affy_med_2011_06 - Legume plants establish a symbiotic interaction with soil bacteria called rhizobia. A complex molecular dialogue between the two partners is necessary for the successful infection and organogenesis processes that will ultimately result in the formation of a nitrogen fixing nodule. During a M. truncatula Tnt1 mutant screen, a new class of symbiotic mutant called noot (nodule root) was identified. This original single recessive mutant develops a root in apical position of nodules that are colonized by bacteria and functional for nitrogen fixation. This conversion suggests that the legume nodule morphogenetic pathway may be derived from a root program. We cloned the NOOT gene and showed that it corresponds to Pisum sativum COCH (Ferguson and Reid, 2005, Plant Cell Physiol 46, 1583-1589). The goal of this project is to compare the transcriptomes from wild type nodules and roots to the noot nodule one. This will allow us to unravel the similarity and differences between the wild type and mutant nodule transcriptomes but also to know which developmental pathway is under the control of the NOOT gene. --Seeds of the WT and noot mutant lines (two mutant alleles: Tnk507 and NF2717) were surface sterilized and placed at 4°C for three days on a minimal BNM medium square plate (12cm X 12cm). Seeds were germinated at room temperature and 10 seeds were placed in a row on nitrogen poor (BNM) plate. Seedlings were inoculated using Sinorhizobium meliloti Rm41 for nodule production. Some WT plants were not infected by the bacteria in order to collect roots. Nodules and roots were harvested 18 to 21 days post inoculation. Each experimental repetition (3 WT nodules, 3 WT roots and 2 mutant nodules of each allele) was harvested at a separated day. RNA preparations were done using a Quiagen Kit.

Series_overall_design:
10 arrays - Medicago; gene knock out,organ comparison

PLEXdb Curator's Note:

KEY:
R108R_1= WT root uninoculated
R108R_2= WT root uninoculated
R108R_10= WT root uninoculated
R108N_1= WT nodule inoculated
R108N_2= WT nodule inoculated
R108N_3= WT nodule inoculated
NOOT_NF2717_1= noot NF2717 nodule inoculated
NOOT_NF2717_2= noot NF2717 nodule inoculated
NOOT_TNKS07_1= noot Tnk507 nodule inoculated
NOOT_TNKS07_2= noot Tnk507 nodule inoculated
Publication: none
Created: 2012-07-26 11:00:23
Last Update: 2012-07-26 12:47:40
Released: 2012-07-31
GEO Accession GSE36558
Submitter: PLEXdb Curator
Name: Stephanie Huguet
Institution: INRA, Evry, France
Head of Laboratory: Claire Lurin
email(s):
Homepage:

 
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