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Browse MedicagoPLEX Experiment Data

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A transcript profile of Medicago truncatula 2HA and Jemmalong during somatic embryogenesis
Peta,,Holmes, Genomic Interactions Group, Canberra, Australia (peta.holmes@anu.edu.au)
Experiment design (6 hybridizations)
genetic line
•2HA •Jemalong

This experiment has been imported by PLEXdb from NCBI GEO (GSE8131)

Series_summary: The Medicago truncatula line 2HA has a 500-fold gre...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: A transcript profile of Medicago truncatula 2HA and Jemmalong during somatic embryogenesis
Accession No: ME3
Microarray: Medicago61k
Visibility: public
Experiment Type:
Experiment Factor(s):
genetic line
•2HA   •Jemalong
Quality Control: biological replicates
Treatment summary:
 genetic line  # replicates
 2HA  3
 Jemalong  3
Total hybridizations: 6
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE8131)

Series_summary:
The Medicago truncatula line 2HA has a 500-fold greater capacity to regenerate plants in culture by somatic embryogenesis than wild-type Jemalong. We have compared transcriptomes of tissue cultures from leaf explants of these two lines. We have used the Affymetrix Medicago Genome Array GeneChip to compare leaf explants of Medicago truncatula Jemalong and the superembryogenic line 2HA after two weeks of tissue culture on 10 µM 1-naphthaleneacetic acid (NAA) and 4 µM 6-benzylaminopurine (BAP) (P4 10:4)


Keywords: Genotype comparison

Series_overall_design:
Leaf explants from the two genotypes were treated with 10:4 for two weeks; total RNA was extracted and used for hybridization to Affymetrix arrays
Publication: 'Genome-wide transcriptional analysis of super-embryogenic Medicago truncatula explant cultures', Imin N, Goffard N, Nizamidin M, Rolfe BG
BMC Plant Biol 2008 Oct 27;8:110
pubmed: 18950541
Created: 2009-07-02 14:42:00
Last Update: 2011-06-28 11:01:23
GEO Accession GSE8131
Submitter: PLEXdb Curator
Name: Peta,,Holmes
Institution: Genomic Interactions Group, Canberra, Australia
Head of Laboratory: --
email(s):
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