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Browse MedicagoPLEX Experiment Data

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A transcript profile of Medicago truncatula 2HA tissue culture with auxin treatment
Peta,Holmes, Genomic Interactions Group, Canberra, Australia (peta.holmes@anu.edu.au)
Experiment design (6 hybridizations)
compound
•NAA •no_NAA

This experiment has been imported by PLEXdb from NCBI GEO (GSE8138)

Series_summary:
Leaf explants of the superembryogenic Medicago t...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: A transcript profile of Medicago truncatula 2HA tissue culture with auxin treatment
Accession No: ME5
Microarray: Medicago61k
Visibility: public
Experiment Type:
Experiment Factor(s):
compound
•NAA   •no_NAA
Quality Control: biological replicates
Treatment summary:
 compound  # replicates
 NAA  3
 no_NAA  3
Total hybridizations: 6
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE8138)

Series_summary:
Leaf explants of the superembryogenic Medicago truncatula line 2HA were treated with auxin (1-naphthaleneacetic acid) for one week to induce the formation of roots (Imin et al J Exp Bot 58:439-451). Gene expression in the leaves and the NAA treated tissue cultures was compared to identify transcripts expressed during the commitment to root formation in tissue culture. We have used the Affymetrix Medicago Genome Array GeneChip to compared gene expression in Medicago truncatula leaves and leaf explants that have been cultured for one week on NAA, to identify genes expressed during the commitment to root formation in tissue culture.

Keywords: Cell type comparison

Series_overall_design:
Medicago truncatula 2HA leaves and leaf explants treated with NAA for one week were collected; total RNA was extracted and used for hybridization to Affymetrix arrays
Publication: none
Created: 2009-07-06 11:18:01
Last Update: 2011-07-15 12:30:56
Released: 2011-07-15
GEO Accession GSE8138
Submitter: PLEXdb Curator
Name: Peta,Holmes
Institution: Genomic Interactions Group, Canberra, Australia
Head of Laboratory: --
email(s):
Homepage:

 
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