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Comparison of transcriptome profile between wild-type and dao mutant plants
Zhao Zhi Gang, Nanjing Agricultural University, Institute of Crop Science, Nanjing, China (zhaozg@njau.edu.cn)
Experiment design (6 hybridizations)
genotype
•wildtype •dao mutant

This experiment has been imported by PLEXdb from NCBI GEO (GSE41097)

Series_summary:
We identified and characterized that the rice Di...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Comparison of transcriptome profile between wild-type and dao mutant plants
Accession No: OS101
Microarray: Rice57k
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•wildtype   •dao mutant
Quality Control: biological replicates
Treatment summary:
 genotype  # replicates
 wildtype  3
 dao mutant  3
Total hybridizations: 6
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE41097)

Series_summary:
We identified and characterized that the rice Dioxygenase for Auxin Oxidation (dao) mutant displays pleiotropic phenotypes, including indehiscent anthers, sterile pollens, and increased free IAA levels in anthers.We conclude that DAO-mediated auxin catabolism is essential for auxin homeostasis and later stages of plant reproductive development, including anther dehiscence, and pollen viability.
We used microarrays to identify differentially expressed genes in dao.

Series_overall_design:
For genome-wide expression analysis of dao, three replicates of WT and dao samples (RNA from mature anthers) were analyzed on Affymetrix Genechip® Rice Genome arrays by an Affymetrix service facility (CapitalBio Corporation) according to the manufacturer’s protocols. Genes showing a 2-fold change with a q-value ≤ 0.05 were considered to be differentially expressed.
Publication: none
Created: 2012-10-30 12:52:50
Last Update: 2012-10-30 14:49:59
Released: 2012-11-01
GEO Accession GSE41097
Submitter: PLEXdb Curator
Name: Zhao Zhi Gang
Institution: Nanjing Agricultural University, Institute of Crop Science, Nanjing, China
Head of Laboratory: --
email(s):
Homepage:

 
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