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Expression information of splicing factor OsSKIPa knock-down and overexpressed rice
Kabin,,Xie, Huazhong Agriculture University, Wuhan, China (xiekabin@webmail.hzau.edu.cn)
Experiment design (6 hybridizations)
genotype
•S58S(overexpressor) •S59R(repressed) •wildtype(control)

This experiment has been imported by PLEXdb from NCBI GEO (GSE10054)

Series_summary:
OsSKIPa is an SNW/SKIP protein in rice, which h...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Expression information of splicing factor OsSKIPa knock-down and overexpressed rice
Accession No: OS18
Microarray: Rice57k
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•S58S(overexpressor)   •S59R(repressed)   •wildtype(control)
Quality Control: biological replicates
Treatment summary:
 genotype  # replicates
 S58S(overexpressor)  2
 S59R(repressed)  2
 wildtype(control)  2
Total hybridizations: 6
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE10054)

Series_summary:
OsSKIPa is an SNW/SKIP protein in rice, which homolog to SKIP(human) and PRP45(yeast). OsSKIPa can rescue the lethal of yeast prp45 mutants. OsSKIPa-suppressed plants (S59R) show decrease of cell viability. Overexpression of OsSKIPa (S58S) increase the osmotic stresses tolerance. OsSKIPa is an important regulator and interacting with a lot of proteins which have varient functions. In order to find the genes expression information when OsSKIPa changed. We use microarray to find changed genes in S59R and S58S plants in the normal condition.

Keywords: gene expression array-based (RNA / in situ oligonucleotide)

Series_overall_design:
Whole plants were collected from 1-month-old S58S (two replicates), S59R (three replicates) and wild type (three replicates).

PLEXdb Curator's Note:
We could found only two replications for each treatment contrary to the 3 reps mentioned at GEO.
Publication: none
Created: 2009-05-15 13:31:04
Last Update: 2009-05-15 13:53:55
GEO Accession GSE10054
Submitter: PLEXdb Curator
Name: Kabin,,Xie
Institution: Huazhong Agriculture University, Wuhan, China
Head of Laboratory: --
email(s):
Homepage:

 
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