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Browse RicePLEX Experiment Data

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Glycinebetaine-induced water-stress tolerance in codA-expressing transgenic indica rice
Akhilesh,Kumar,Tyagi, University of Delhi South Campus, Benito Juarez Road, India: Department of Plant Molecular Biology (akhilesh@genomeindia.org)
Experiment design (9 hybridizations)
genetic line
•Transgenic line 4 •Transgenic line 22 •Wild-type

This experiment has been imported by PLEXdb from NCBI GEO (GSE15448)

Series_summary:
Rice (Oryza sativa L.), a non-accumulator of gly...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Glycinebetaine-induced water-stress tolerance in codA-expressing transgenic indica rice
Accession No: OS27
Microarray: Rice57k
Visibility: public
Experiment Type:
Experiment Factor(s):
genetic line
•Transgenic line 4   •Transgenic line 22   •Wild-type
Quality Control:
Treatment summary:
 genetic line  # replicates
 Transgenic line 4  3
 Transgenic line 22  3
 Wild-type  3
Total hybridizations: 9
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE15448)

Series_summary:
Rice (Oryza sativa L.), a non-accumulator of glycinebetaine (GB), is highly susceptible to abiotic stress. Transgenic rice with chloroplast-targeted choline oxidase encoded by the codA gene from Arthrobacter globiformis has been evaluated for inheritance of transgene up to R5 generation and water-stress tolerance. During seedling, vegetative and reproductive stages, transgenic plants could maintain higher activity of photosystem II and they show better physiological performance, e.g. enhanced detoxification of reactive oxygen species compared to wild-type plants under water-stress. Survival rate and agronomic performance of transgenic plants is also better than wild-type following prolonged water-stress. Choline oxidase converts choline into glycinebetaine and H2O2 in a single step. It is possible that H2O2 /GB might activate stress response pathways and prepare transgenic plants to mitigate stress. To check this possibility, microarray-based transcriptome analysis of transgenic rice has been done. It unraveled altered expression of many genes involved in stress responses, signal transduction, gene regulation, hormone signaling and cellular metabolism. Overall, 165 genes show more than 2 folds up-regulation at P value <0.01 in transgenic rice. Out of these, at least 50 genes are known to be involved in plant stress response. Exogenous application of H2O2 or GB to wild-type plants also induces such genes. Our data show that metabolic engineering for GB is a promising strategy for introducing stress tolerance in crop plants and which could be imparted, in part, by H2O2- and/or GB-induced stress response genes.

Series_overall_design:
Rice (Oryza sativa L.), transgenic plants expressing codA gene from Arthrobacter globiformis were compared with untransformed plants at seedling level
Publication: 'Glycinebetaine-induced water-stress tolerance in codA-expressing transgenic indica rice is associated with up-regulation of several stress responsive genes.', Kathuria H, Giri J, Nataraja KN, Murata N et al.
Plant Biotechnol J 2009 Aug;7(6):512-26
pubmed: 19490479
Created: 2010-02-01 14:44:50
Last Update: 2010-02-02 12:41:35
GEO Accession GSE15448
Submitter: PLEXdb Curator
Name: Akhilesh,Kumar,Tyagi
Institution: University of Delhi South Campus, Benito Juarez Road, India: Department of Plant Molecular Biology
Head of Laboratory: --
email(s):
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