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Browse RicePLEX Experiment Data

Choose an experiment:
Rice seedling hormone treatment
Mukesh,,Jain, National Institute for Plant Genome Research (NIPGR) (mjainanid@gmail.com)
Experiment design (6 hybridizations)
treatment
•control •IAA treated •BAP treated

This experiment has been imported by PLEXdb from NCBI GEO (GSE5167)

Series_summary:
Indica rice seedlings of IR64 variety were grown ...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Rice seedling hormone treatment
Accession No: OS28
Microarray: Rice57k
Visibility: public
Experiment Type:
Experiment Factor(s):
treatment
•control   •IAA treated   •BAP treated
Quality Control: biological replicates
Treatment summary:
 treatment  # replicates
 control  2
 IAA treated  2
 BAP treated  2
Total hybridizations: 6
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE5167)

Series_summary:
Indica rice seedlings of IR64 variety were grown hydroponically for 7-days in a culture room with a daily photoperiodic cycle of 14h light and 10h dark. Seedlings were incubated in 0.1% dimethyl sulfoxide (control) or 50 micromolar solutions of indole-3-acetic acid (IAA treatment) and benzyl aminopurine (BAP treatment) for 1h and 3h. Equal amounts of 1h and 3h samoles were pooled for each treatment before RNA isolation. The 5 micrograms of each total RNA sample was processed for microarray analysis according to Affymetrix protocol.
Keywords: Rice, seedling, IAA, BAP, hormone response

Series_overall_design:
Two biological replicates for each sample (control, IAA treated, BAP treated) were used for microarray analysis. The experiment involves the identification of differentially expressed genes in IAA and BAP treated samples as compared to control.
Publication: 'Transcript profiling reveals diverse roles of auxin-responsive genes during reproductive development and abiotic stress in rice.', Jain M, Khurana JP.
FEBS J 2009 Jun;276(11):3148-62.
pubmed: 19490115
Created: 2010-02-16 13:44:52
Last Update: 2010-02-16 14:26:56
GEO Accession GSE5167
Submitter: PLEXdb Curator
Name: Mukesh,,Jain
Institution: National Institute for Plant Genome Research (NIPGR)
Head of Laboratory: --
email(s):
Homepage:

 
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