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Temporal gene expression analyisis from rice root (cv. Nipponbare) infected with Magnaporthe oryzae strain Guy11
Sylvain,,Marcel, University of Lausanne, Department of Plant Molecular Biology, Grass Arbuscular Mycorrhiza Group, Campus Dorigny, Lausanne, VD, 1015, Switzerland (not given in GEO)
Experiment design (12 hybridizations)
pathogen inoculation
•Mock •Guy11_inoculated
time
•2 dpi •4 dpi •6 dpi

This experiment has been imported by PLEXdb from NCBI GEO (GSE18361)

Series_summary:
Magnaporthe oryzae causes rice blast, the most d...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Temporal gene expression analyisis from rice root (cv. Nipponbare) infected with Magnaporthe oryzae strain Guy11
Accession No: OS35
Microarray: Rice57k
Visibility: public
Experiment Type:
Experiment Factor(s):
pathogen inoculation
•Mock   •Guy11_inoculated
time
•2 dpi  •4 dpi  •6 dpi
Quality Control:
Treatment summary:
 pathogen inoculation  time  # replicates
 Mock  2 dpi  2
 Mock  4 dpi  2
 Mock  6 dpi  2
 Guy11_inoculated  2 dpi  2
 Guy11_inoculated  4 dpi  2
 Guy11_inoculated  6 dpi  2
Total hybridizations: 12
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE18361)

Series_summary:
Magnaporthe oryzae causes rice blast, the most devastating foliar fungal disease of cultivated rice. During disease development the fungus simultaneously maintains both biotrophic and necrotrophic growth corresponding to a hemi-biotrophic life style. The ability of M. oryzae to also colonize roots and subsequently develop blast symptoms on aerial tissue has been recognized. The fungal root infection strategy and the respective host responses are currently unknown. Global temporal expression analysis suggested a purely biotrophic infection process reflected by the rapid induction of defense response-associated genes at the early stage of root invasion and subsequent repression coinciding with the onset of intracellular fungal growth. The same group of down-regulated defense genes was increasingly induced upon leaf infection by M. oryzae where symptom development occurs shortly post tissue penetration. Our molecular analysis therefore demonstrates the existence of fundamentally different tissue-specific fungal infection strategies and provides the basis for enhancing our understanding of the pathogen life style.

Series_overall_design:
We investigated global transcriptome response overtime of Mock- and M. oryzae inoculated rice root tissue in vitro. Two independant replicates were perfomed for each treatments and samples were collected at 2, 4 and 6 days post-inoculation.
Publication: none
Created: 2010-03-12 14:07:52
Last Update: 2010-03-12 14:43:11
Released: 2010-04-02
GEO Accession GSE18361
Submitter: PLEXdb Curator
Name: Sylvain,,Marcel
Institution: University of Lausanne, Department of Plant Molecular Biology, Grass Arbuscular Mycorrhiza Group, Campus Dorigny, Lausanne, VD, 1015, Switzerland
Head of Laboratory: --
email(s):
Homepage:

 
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