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Browse RicePLEX Experiment Data

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Genome-wide temporal-spatial gene expression profiling of drought responsiveness in rice
Pan Yajiao, The Chinese Academy of Agriculture Sciences, Beijing, China (panyajiao@yahoo.com.cn,fuby@caas.net.cn,lizhk@caas.net.cn)
Experiment design (36 hybridizations)
stress condition
•control •drought
tissue type name
•leaves •roots •panicle
developmental stage name
•tillering stage •panicle elongation stage •booting stage

This experiment has been imported by PLEXdb from NCBI GEO (GSE26280) Series_summary: Rice is highly sensitive to drought, and the effect of dr...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Genome-wide temporal-spatial gene expression profiling of drought responsiveness in rice
Accession No: OS37
Microarray: Rice57k
Visibility: public
Experiment Type:
Experiment Factor(s):
stress condition
•control   •drought
tissue type name
•leaves   •roots   •panicle
developmental stage name
•tillering stage   •panicle elongation stage   •booting stage
Quality Control: biological replicates
Treatment summary:
 stress condition  tissue type name  developmental stage name  # replicates
 control  leaves  tillering stage  3
 control  leaves  panicle elongation stage  3
 control  leaves  booting stage  3
 control  roots  tillering stage  3
 control  roots  panicle elongation stage  3
 control  roots  booting stage  0
 control  panicle  tillering stage  0
 control  panicle  panicle elongation stage  0
 control  panicle  booting stage  3
 drought  leaves  tillering stage  3
 drought  leaves  panicle elongation stage  3
 drought  leaves  booting stage  3
 drought  roots  tillering stage  3
 drought  roots  panicle elongation stage  3
 drought  roots  booting stage  0
 drought  panicle  tillering stage  0
 drought  panicle  panicle elongation stage  0
 drought  panicle  booting stage  3
Total hybridizations: 36
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE26280)

Series_summary:
Rice is highly sensitive to drought, and the effect of drought may vary with the different genotypes and development stages. Genome-wide gene expression profiling was used as the initial point to dissect molecular genetic mechanism of this complex trait and provide valuable information for the improvement of drought tolerance in rice. Affymetrix rice genome array containing 48,564 japonica and 1,260 indica sequences was used to analyze the gene expression pattern of rice exposed to drought stress. The transcriptome from leaf, root, and young panicle at three developmental stages was comparatively analyzed combined with bioinformatics exploring drought stress related cis-elements.

Series_overall_design:
In this study, the gene expression patterns across six tissues including leaves and roots at tillering stage and panicle elongation stage, leaves and young panicle at booting stage ( TL: leaves at tillering stage; TR: roots at tillering stage; PL: leaves at panicle elongation stage; PR: roots at panicle elongation stage; BP: young panicle at booting stage; BL: leaves at booting stage) were characterized by using the Affymetrix rice microarray platform based on a drought tolerant rice line derived from IR64.
Publication: none
Created: 2011-03-04 13:01:49
Last Update: 2011-03-08 11:18:37
Released: 2011-03-15
GEO Accession GSE26280
Submitter: PLEXdb Curator
Name: Pan Yajiao
Institution: The Chinese Academy of Agriculture Sciences, Beijing, China
Head of Laboratory: Bin-Ying Fu and Zhi-Kang Li
email(s):
Homepage:

 
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