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Browse RicePLEX Experiment Data

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Resistance gene-dependent responses in rice to the bacterial blight pathogen Xanthomonas oryzae pv. oryzae
David O Nino-Liu, Plant Pathology Department, Iowa State University (david.o.nino-liu@monsanto.com, ajbog@iastate.edu)
Experiment design (45 hybridizations)
genotype
•IR24 •IRBB5 •IRBB7
time
•0 hai •3 hai •6 hai •12 hai •24 hai

TITLE: Transcriptional profiling of Rgene-mediated responses in rice PROJECT DESCRIPTION: The dominant gene Xa7 and the recessive gene xa5 of r...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Resistance gene-dependent responses in rice to the bacterial blight pathogen Xanthomonas oryzae pv. oryzae
Accession No: OS4
Microarray: Rice57k
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•IR24   •IRBB5   •IRBB7
time
•0 hai  •3 hai  •6 hai  •12 hai  •24 hai
Quality Control: biological replicates
Treatment summary:
 genotype  time  # replicates
 IR24  0 hai  3
 IR24  3 hai  3
 IR24  6 hai  3
 IR24  12 hai  3
 IR24  24 hai  3
 IRBB5  0 hai  3
 IRBB5  3 hai  3
 IRBB5  6 hai  3
 IRBB5  12 hai  3
 IRBB5  24 hai  3
 IRBB7  0 hai  3
 IRBB7  3 hai  3
 IRBB7  6 hai  3
 IRBB7  12 hai  3
 IRBB7  24 hai  3
Total hybridizations: 45
Description: TITLE: Transcriptional profiling of Rgene-mediated responses in rice

PROJECT DESCRIPTION: The dominant gene Xa7 and the recessive gene xa5 of
rice confer resistance to several races of the bacterial blight pathogen
Xanthomonas oryzae pv. oryzae (Xoo). To reveal the modes of action and
the defense responses these genes initiate, we decided to obtain the
global transcriptional profiles of the rice cultivars IRBB7, IRBB5
(which harbor Xa7 and xa5, respectively) and IR24 undergoing early
infection by the Xoo Race 2 strain PXO86. Both IRBB7 and IRBB5 are
resistant to PXO86 (which carry the corresponding avirulence genes
avrXa7 and avrxa5), whereas IR24 is susceptible. We inoculated by vacuum
infiltration the three rice cultivars ten days after seed germination
(or 2 weeks after sowing) and collected inoculated tissue at 5 different
timepoints within the first day after inoculation. The transcriptional
profiles obtained will provide valuable insight into the similarities
and differences between incompatible interactions mediated by a dominant
and a recessive Rgene, in comparison to a compatible interaction.

Submitted at NCBI GEO via PLEXdb.

Publication: none
Created: 2008-03-21 15:13:06
Last Update: 2008-04-17 22:32:27
GEO Accession GSE33411
Submitter: David O Nino-Liu
Name: David O Nino-Liu
Institution: Plant Pathology Department, Iowa State University
Head of Laboratory: Adam J Bogdanove
email(s):
Homepage:

 
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