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Experiment Name:

Transcriptional profiling of Cellulase induced hypersensitive reaction like response in rice

Accession No:

OS40

Microarray:

Rice57k

Visibility:

public

Experiment Type:

Experiment Factor(s):

compound: •potassium phosphate buffer •Xoo cellulase

Quality Control:

biological replicates

Treatment summary:

compound	# replicates
potassium phosphate buffer	3
Xoo cellulase	3

Total hybridizations: 6

Description:

This experiment has been imported by PLEXdb from NCBI GEO (GSE8216)

Series_summary:
Cellulase, a Type II secretion system secreted protein of Xanthomonas oryzae pv. oryzae (Xoo; the casual of bacterial leaf blight of rice) is a potent inducer of rice defense responses such as hypersensitive response like reactions (HR), callose depositions, cell death associated with nuclear fragmentations and impart functional resistance against further Xoo inoculation
In order to understand the molecular events associated with cellulase induced HR in rice, whole genome transcriptional profiling was performed using Affymetrix Rice GeneChips
Keywords: Expression profiling of a hypersensitive reaction like response

Series_overall_design:
Leaves of 20 days old green house grown susceptible rice cultivar (Taichung Native-1; TN-1) were infiltrated with either 30-40μl of purified Xoo cellulase (5μg/ml) or with buffer (10mM potassium phosphate buffer pH 6.0) alone (as described in Jha et al. 2007; MPMI vol 20, pp 31-40). The plants were shifted to a growth chamber (28oC; 80% relative humidity; 12/12h light/dark cycle) 48 hours before the treatment. 20-30 leaf pieces covering the infiltrated zone from each of the treatments were harvested 12 h after infiltration. Total RNA isolated from the pooled samples was subjected for expression analysis using Affymetrix GeneChip System. The experiment was repeated with three different biological replicates using RNA isolated from three batches of rice leaves treated with the freshly purified Xoo cellulase and the buffer

Publication:

'Transcriptional profiling of rice leaves undergoing a hypersensitive response like reaction induced by Xanthomonas oryzae pv. oryzae cellulase.', Gopaljee Jha, Hitendra Kumar Patel, Madhumita Dasgupta, Ramesh Palaparthi and R. V. Sonti.
Rice 2010. 3: 1-21. DOI: 10.1007/s12284-009-9033-z

Created:

2011-03-22 11:50:06

Last Update:

2011-03-22 12:10:36

Released:

2011-03-23

GEO Accession

GSE8216

Submitter:

PLEXdb Curator

Experimenter:

Name:	Ramesh V. Sonti
Institution:	Centre for cellular and molecular biology (CCMB), Department of Plant Microbe Interactions, Hyderabad, India
Head of Laboratory:	Ramesh V. Sonti
email(s):	sonti@ccmb.res.in
Homepage:


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