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Temporal regulatory role of miR156 during leaf ontogenesis
Kabin Xie, Huazhong Agriculture University, National Key Laboratory of Crop Genetic Improvement, Wuhan, China (xiekabin@webmail.hzau.edu.cn,lizhongx@mail.hzau.edu.cn)
Experiment design (12 hybridizations)
tissue type name
•younger leaves(L1) •older leaves(L4-5)
genotype
•miR156 •wildtype

This experiment has been imported by PLEXdb from NCBI GEO (GSE14692) Series_summary: Analysis of different leaves from transgenic plants over e...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Temporal regulatory role of miR156 during leaf ontogenesis
Accession No: OS48
Microarray: Rice57k
Visibility: public
Experiment Type:
Experiment Factor(s):
tissue type name
•younger leaves(L1)   •older leaves(L4-5)
genotype
•miR156   •wildtype
Quality Control: biological replicates
Treatment summary:
 tissue type name  genotype  # replicates
 younger leaves(L1)  miR156  3
 younger leaves(L1)  wildtype  3
 older leaves(L4-5)  miR156  3
 older leaves(L4-5)  wildtype  3
Total hybridizations: 12
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE14692)

Series_summary:
Analysis of different leaves from transgenic plants over expressing microRNA miR156. Time-dependent gene expression is essential for developmental timing control. MiR156 was characterized as a time-dependent expressed microRNA during leaf development. The level of miR156 is positive correlated to developmental time during leaf growth. We used microarray to identify genes regulated by miR156 in a time-dependent manner during leaf development. Results indicate that most time-negative genes were activated in mature leaves of miR156 over expressed plants compared with wild type.

Keywords: gene expression array-based (RNA / in situ oligonucleotide)

Series_overall_design:
To identify the temporal regulation role of miR156 in different leaves, all leaves in the main tiller were collected separately from Md/Mh (miR156 over expressed lines) and wild type (WT) plants. The level of miR156 were checked in these samples, then three biological repeats were selected and these RNA samples of the oldest (L1) and youngest leaf (L4 or L5) were applied for microarray hybridization.
Publication: none
Created: 2011-03-29 13:31:07
Last Update: 2011-03-29 13:44:55
Released: 2011-02-14
GEO Accession GSE14692
Submitter: PLEXdb Curator
Name: Kabin Xie
Institution: Huazhong Agriculture University, National Key Laboratory of Crop Genetic Improvement, Wuhan, China
Head of Laboratory: Lizhong Xiong
email(s):
Homepage:

 
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