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Browse RicePLEX Experiment Data

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bulked RILs with high and low grain number per panicle derived from 2 cultivars at panicle primordia stage
Rupesh Kailasrao Deshmukh, National Research Center on Plant Biotechnology, Rice Genome Laboratory, New Delhi, India (rupdeshmukh@rediffmail.com,nksingh@nrcpb.org)
Experiment design (4 hybridizations)
genetic line
•bulkRIL(high grain no) •bulkRIL(low grain no)

This experiment has been imported by PLEXdb from NCBI GEO (GSE17169) Series_summary: Whole genome transcriptome profiling of bulked RILs with ...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: bulked RILs with high and low grain number per panicle derived from 2 cultivars at panicle primordia stage
Accession No: OS51
Microarray: Rice57k
Visibility: public
Experiment Type:
Experiment Factor(s):
genetic line
•bulkRIL(high grain no)   •bulkRIL(low grain no)
Quality Control: biological replicates
Treatment summary:
 genetic line  # replicates
 bulkRIL(high grain no)  2
 bulkRIL(low grain no)  2
Total hybridizations: 4
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE17169)

Series_summary:
Whole genome transcriptome profiling of bulked RILs with high and low grain number per panicle derived from 2 cultivars at panicle primordia stage

The aim of this study was to identify candidate genes responsible for grain number per panicle by combining QTLs analysis with expression analysis. Microarray analysis of RNA extracted from the panicle primordia showed 20 differentially expressed genes, respectively. The differentially expressed genes were shorted to 4 on the basis of their occurance in the QTL region (responcible for grain number regulation) detected in RIL population derived from Pusa 1266 and Pusa Basmati 1.

Series_overall_design:
RNA from the stage '0' panicle primordia of 10 RILs with high grain number and 10 with low grain number were bulked and analysed in two different biological replications (A and B) making total four samples
Publication: none
Created: 2011-04-01 11:08:18
Last Update: 2011-04-01 11:22:57
Released: 2011-02-14
GEO Accession GSE17169
Submitter: PLEXdb Curator
Name: Rupesh Kailasrao Deshmukh
Institution: National Research Center on Plant Biotechnology, Rice Genome Laboratory, New Delhi, India
Head of Laboratory: Nagendra K. Singh
email(s):
Homepage:

 
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