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Browse RicePLEX Experiment Data

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Comparison of transcriptional responses of two susceptible rice cultivars to strains of Xanthomonas oryzae
Ginny Antony, Kansas State University (ginns@ksu.edu)
Experiment design (65 hybridizations)
cultivar
•Nipponbare •IR24
strain
•PXO99A •PXO99AME7 •PXO99AME5 •MOCK •NO TREATMENT •T7174 •PXO86 •BLS303 •PXO99AME1 •PXO99AME2 •PXO99A-2

X. oryzae pv. oryzae (Xoo) is the causal agent of bacterial blight of rice. X. oryzae pv. oryzicola (Xoc) is the causal agent of bacterial streak ...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Comparison of transcriptional responses of two susceptible rice cultivars to strains of Xanthomonas oryzae
Accession No: OS66
Microarray: Rice57k
Visibility: public
Experiment Type:
Experiment Factor(s):
cultivar
•Nipponbare   •IR24
strain
•PXO99A   •PXO99AME7   •PXO99AME5   •MOCK   •NO TREATMENT   •T7174   •PXO86   •BLS303   •PXO99AME1   •PXO99AME2   •PXO99A-2
Quality Control: biological replicates
Treatment summary:
 cultivar  strain  # replicates
 Nipponbare  PXO99A  3
 Nipponbare  PXO99AME7  3
 Nipponbare  PXO99AME5  3
 Nipponbare  MOCK  3
 Nipponbare  NO TREATMENT  2
 Nipponbare  T7174  3
 Nipponbare  PXO86  3
 Nipponbare  BLS303  3
 Nipponbare  PXO99AME1  3
 Nipponbare  PXO99AME2  3
 Nipponbare  PXO99A-2  3
 IR24  PXO99A  3
 IR24  PXO99AME7  3
 IR24  PXO99AME5  3
 IR24  MOCK  3
 IR24  NO TREATMENT  3
 IR24  T7174  3
 IR24  PXO86  3
 IR24  BLS303  3
 IR24  PXO99AME1  3
 IR24  PXO99AME2  3
 IR24  PXO99A-2  3
Total hybridizations: 65
Description: X. oryzae pv. oryzae (Xoo) is the causal agent of bacterial blight of rice. X. oryzae pv. oryzicola (Xoc) is the causal agent of bacterial streak of rice. Fourteen day old rice leaves were inoculated with one of five strains of Xanthomonas oryzae. Seven strains of Xoo were used; three wild type strains (PXO99A, T7174, and PXO86) and strains PXO99AME7, which has a nonfunctional type III secretion system and is non-pathogenic, , PXO99AME1, a pthXo6 and avrXa27 double mutant, PXO99ME2, a pthXo1 mutant, and PXO99ME5, a reduced virulence strain with uncharacterized mutation in a TAL effector. One strain of Xoc (BLS303) was tested. Controls include an inoculation with water (MOCK) and no inoculation. T7174 is our label for the Japanese isolate MAFF311018. Third leaves are inoculated with a needless syringe at adjacent sites along the upper leaf blade. Six leaves from separate plants are pooled. RNA samples were collected 24 h after treatment.
PLEXdb Curator's Note:
(Original submission was OS59 whic is now deleted.)
Submitted at GEO via PLEXdb.

Publication: none
Created: 2011-09-15 00:00:00
Last Update: 2012-03-01 14:45:33
Released: 2012-03-01
GEO Accession GSE36272
Submitter: Frank White
Name: Ginny Antony
Institution: Kansas State University
Head of Laboratory: Frank White
email(s):
Homepage:

 
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