Search   for 
Find Your Gene ·  Publications ·  Tools ·  Gene List Suite ·  Expression Atlases ·  About PLEXdb ·  Feedback
Login ID:
Password:
Plants
Arabidopsis
Barley
Brachypodium
Citrus
Cotton
Grape
Maize
Medicago
Poplar
Rice
Soybean
Sugarcane
Tomato
Wheat
Pathogens & Symbionts
Search Experiments
Download Data
Gene List Suite
Tools
Your Account
Documentation
News
Personnel
Links
 Data Release Policy 
PLEXdb is committed to making expression data publicly available to researchers world-wide...
complete policy
 

Browse RicePLEX Experiment Data

Choose an experiment:
affy_meloidogyne_rice2-Oryza sativa genes expressed in the early compatible interaction with root-knot nematode
Stephanie Huguet, INRA, Evry, France (huguet@evry.inra.fr)
Experiment design (6 hybridizations)
pathogen inoculation
•control •inoculated

This experiment has been imported by PLEXdb from NCBI GEO (GSE21772)

Series_summary:
affy_meloidogyne_rice2 - affy_meloidogyne_rice2 -...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

Show
Experiment Name: affy_meloidogyne_rice2-Oryza sativa genes expressed in the early compatible interaction with root-knot nematode
Accession No: OS70
Microarray: Rice57k
Visibility: public
Experiment Type:
Experiment Factor(s):
pathogen inoculation
•control   •inoculated
Quality Control: biological replicates
Treatment summary:
 pathogen inoculation  # replicates
 control  3
 inoculated  3
Total hybridizations: 6
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE21772)

Series_summary:
affy_meloidogyne_rice2 - affy_meloidogyne_rice2 - Plant-parasitic nematodes cause heavy economic losses to global agriculture. The root knot nematode, Meloidogyne incognita, is an obligate parasite that causes significant damage to a broad range of host plants. M. incognita infection to dicotyledous plants is extensively studied but it is also important to study their interaction with monocotyledous plants, in particular with cereals. In our growing conditions, as of day 6, histological studies revealed a profound rice tissue reorganisation around nematodes, notably characterized by the plant feeding site formation. We are investigating the molecular plant response to M. incognita by carrying out a global analysis of gene expression during gall formation in rice, using giant cell-enriched root tissues at this early stage (6dpi) of gall development-Oryza sativa (var. Nipponbare) seedlings were grown on 6 cm3 SAP substrate completed with diluted Hoaglands solution (Reversat et al., 1999). Culture units were placed in a growth chamber illuminated with fluorescent tubes 9/24 h and maintained at 23°C for 6 days before being inoculated with a 300 J2-stage juveniles M. incognita. One day after inoculation (dai), the rice seedlings were immersed in de-ionised water to remove all J2s that had not penetrated the roots and allowing synchronization of the infection. Each seedling was transferred to a hydroponic mini chamber (Reversat et al., 2004). Sampling was performed at 6 dai and each of them contained galls from 45 infected plants, they were then hand-dissected, frozen in liquid-nitrogen and stored at -80°C. As reference samples, uninfected meristematic root fragments were dissected from seedlings grown under the same conditions. Each sample was replicated 3 times.
Keywords: normal vs disease comparison

Series_overall_design:
6 arrays - rice
Publication: none
Created: 2011-11-03 10:54:42
Last Update: 2011-11-03 14:47:03
Released: 2011-11-03
GEO Accession GSE21772
Submitter: PLEXdb Curator
Name: Stephanie Huguet
Institution: INRA, Evry, France
Head of Laboratory: Claire Lurin
email(s):
Homepage:

 
Copyright@2001-2013 The PLEXdb Group
All rights reserved.

For problems with the webpages contact PLEXdb webmasters