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Browse RicePLEX Experiment Data

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A single functional blast resistance gene Pi54 activates complex defense mechanism in Rice
Dr. Tilak Raj Sharma, National Research Centre on Plant Biotechnology, Department of Biotechnology, New Dehli, India (trsharma@nrcpb.org)
Experiment design (8 hybridizations)
genotype
•TP •TP-Pi54
pathogen inoculation
•control •inoculated

This experiment has been imported by PLEXdb from NCBI GEO (GSE28308)

Series_summary:
To investigate the responses of resistance gene ...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: A single functional blast resistance gene Pi54 activates complex defense mechanism in Rice
Accession No: OS79
Microarray: Rice57k
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•TP   •TP-Pi54
pathogen inoculation
•control   •inoculated
Quality Control: biological replicates
Treatment summary:
 genotype  pathogen inoculation  # replicates
 TP  control  2
 TP  inoculated  2
 TP-Pi54  control  2
 TP-Pi54  inoculated  2
Total hybridizations: 8
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE28308)

Series_summary:
To investigate the responses of resistance gene Pi54 at transcriptome level in blast resistant transgenic rice line TP-Pi54 during infection by Magnaporthe oryzae, we studied the co-expression analysis using microarray along with susceptible non-transgenic line Taipei 309 (TP). Suitable controls for both lines were used. The comparative analysis of differentially expressed genes was conducted.

Series_overall_design:
Total RNA from the leaves of mock inoculated non transgenic line Taipei 309 (TP) and transgenic line (TP-Pi54) along with challenge inoculated plants were isolated at 72 hours post inoculation. The samples were analyzed in two different biological replications (A and B) making total eight samples.


PLEXdb Curator's Note:
The PO terms at PLEXdb reflect the seedling tissues as mentioned in the GEO sample records, as opposed to, leaves mentioned in the GEO experiment description page.
Publication: 'The single functional blast resistance gene Pi54 activates a complex defence mechanism in rice.', Gupta SK, Rai AK, Kanwar SS, Chand D, Singh NK, Sharma TR.
J Exp Bot 2012 Jan;63(2):757-72.
pubmed: 22058403
Created: 2012-02-28 10:24:58
Last Update: 2012-03-01 10:50:55
Released: 2012-03-01
GEO Accession GSE28308
Submitter: PLEXdb Curator
Name: Dr. Tilak Raj Sharma
Institution: National Research Centre on Plant Biotechnology, Department of Biotechnology, New Dehli, India
Head of Laboratory: Dr. Tilak Raj Sharma
email(s):
Homepage:

 
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