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Expression data from rice after brown planthopper attack
Yubing Wang, Institute of Plant Physiology and Ecology, Shanghai, China (ybwang@sippe.ac.cn)
Experiment design (18 hybridizations)
cultivar
•RHT •TN1
time
•0 hrs •8 hrs •24 hrs

This experiment has been imported by PLEXdb from NCBI GEO (GSE29967)

Series_summary:
The aim of this study was to analyze potential br...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Expression data from rice after brown planthopper attack
Accession No: OS82
Microarray: Rice57k
Visibility: public
Experiment Type:
Experiment Factor(s):
cultivar
•RHT   •TN1
time
•0 hrs  •8 hrs  •24 hrs
Quality Control: biological replicates
Treatment summary:
 cultivar  time  # replicates
 RHT  0 hrs  3
 RHT  8 hrs  3
 RHT  24 hrs  3
 TN1  0 hrs  3
 TN1  8 hrs  3
 TN1  24 hrs  3
Total hybridizations: 18
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE29967)

Series_summary:
The aim of this study was to analyze potential brown planthopper (BPH) resistant genes in Rathu Heenati (RHT) by Affymetrix whole rice genome array,BPH susceptible and resistant rice varieties of TN1(Taichung Native 1)as control. All the resistant related genes derived from RHT will be analyzed according to the SSR markers interval flanked on the chromosome 3, 4, 6 and 10. It will be benefit to the gene clone and marker assistant breeding for Bph3 gene in the near future.
We used microarrays to detail the global differential gene expression before and after brown planthopper attack in two different varieties, and identified distinct classes of high enriched genes induced by BPH or constituent in RHT

Series_overall_design:
The 2nd to 3rd instar nymphs of BPH were transferred to tillering stage seedings (10 BPH nymphs per plant) in a box covered with nylon-mesh. Stems of the rice plant infected by BPH were collected at 0h (T0), 8h (T8), 24h (T24) after BPH attack, total RNA were extracted for the microarray hybirdlization.
Publication: none
Created: 2012-03-08 11:26:52
Last Update: 2012-03-08 16:39:58
Released: 0000-00-00
GEO Accession GSE29967
Submitter: PLEXdb Curator
Name: Yubing Wang
Institution: Institute of Plant Physiology and Ecology, Shanghai, China
Head of Laboratory: Xue-Xia Miao
email(s):
Homepage:

 
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