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Rice gene global expression analysis upon inoculation with different Magnaporthe isolates
Pamela Abbruscato, Parco Technologico Padano, Department of Rice Genomics Unit, Lodi, Italy (pamela.abbruscato@tecnoparco.org)
Experiment design (15 hybridizations)
pathogen inoculation
•BR29 •BR32 •CL367 •FR13 •mock

This experiment has been imported by PLEXdb from NCBI GEO (GSE30941)

Series_summary:
Magnaporthe oryzae is the causative agent of the ...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Rice gene global expression analysis upon inoculation with different Magnaporthe isolates
Accession No: OS91
Microarray: Rice57k
Visibility: public
Experiment Type:
Experiment Factor(s):
pathogen inoculation
•BR29   •BR32   •CL367   •FR13   •mock
Quality Control: biological replicates
Treatment summary:
 pathogen inoculation  # replicates
 BR29  3
 BR32  3
 CL367  3
 FR13  3
 mock  3
Total hybridizations: 15
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE30941)

Series_summary:
Magnaporthe oryzae is the causative agent of the rice blast, the most relevant rice disease worldwide. To date expression analysis on rice infected with Magnaporthe oryzae have been carried out only with the strains FR13 (leaf) and Guy 11 (root). However different strains of Magnaporthe are present in the environment leading to different rice responses at molecular level. To gain more insight on the unknown molecular mechanisms activated by different Magnaporthe strains during rice defense, a global expression analysis was performed by using the GeneChip® Rice Genome Array.
To identify rice genes differentially regulated upon infection by Magnaporthe isolates, inoculation with different strains were performed and samples were collected 24 hours post infection.

Series_overall_design:
RNA were obtained from leaf samples after inoculation of rice 2 week-old plantlets with the following strains: rice isolates Magnaporthe oryzae FR13 and CL367, non-adapted strain BR32, isolated from wheat, and Magnaporthe grisea BR29 isolated from crabgrass. Treated and control (mock) rice leaves (cv. Nipponbare) were collected 24 hours post inoculation. Three biological replicates for each interaction type and the corresponding mock were extracted and analysed independently with the GeneChip® Rice Genome Array.
Publication: 'OsWRKY22, a monocot WRKY gene, plays a role in the resistance response to blast.', Abbruscato P, Nepusz T, Mizzi L, Del Corvo M, Morandini P, Fumasoni I, Michel C, Paccanaro A, Guiderdoni E, Schaffrath U, Morel JB, Piffanelli P, Faivre-Rampant O.
Mol Plant Pathol 2012 Mar 23.
pubmed: 22443363
Created: 2012-04-26 09:52:24
Last Update: 2012-04-26 13:12:48
Released: 2012-05-01
GEO Accession GSE30941
Submitter: PLEXdb Curator
Name: Pamela Abbruscato
Institution: Parco Technologico Padano, Department of Rice Genomics Unit, Lodi, Italy
Head of Laboratory: Pamela Abbruscato
email(s):
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