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Browse RicePLEX Experiment Data

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Rice gene global expression during nonhost interaction with Blumeria graminis f. sp. hordei (Bgh)
Pamela Abbruscato, Parco Technologico Padano, Department of Rice Genomics Unit, Lodi, Italy (pamela.abbruscato@tecnoparco.org)
Experiment design (6 hybridizations)
pathogen infection
•Bgh •mock

This experiment has been imported by PLEXdb from NCBI GEO (GSE30942)

Series_summary:
Powdery mildew is a very common plant disease an...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Rice gene global expression during nonhost interaction with Blumeria graminis f. sp. hordei (Bgh)
Accession No: OS92
Microarray: Rice57k
Visibility: public
Experiment Type:
Experiment Factor(s):
pathogen infection
•Bgh   •mock
Quality Control: biological replicates
Treatment summary:
 pathogen infection  # replicates
 Bgh  3
 mock  3
Total hybridizations: 6
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE30942)

Series_summary:
Powdery mildew is a very common plant disease and only few plants are immune. Host interactions have been identified and characterized for the pathosystems barley-B. graminis f. sp. tritici (Bgt) and wheat-B. graminis f. sp. hordei (Bgh), whereas no data are reported about powdery mildew and nonhost plants, such as rice. On the other hand rice nonhost resistance is widely unexploited and only few expression data are available. To characterize rice response during nonhost interaction with Bgh, a global expression analysis was performed by using the GeneChip® Rice Genome Array.
To describe rice gene expression profiles during nonhost interaction, 2 week-old rice plantlets were inoculated with Bgh. Treated (inoculated) and control (mock) samples were collected 24 hours post-inoculation for GeneChip® Rice Genome Array hybridization.

Series_overall_design:
For transcript profiling experiments with powdery mildew, entire leaves were sampled from rice plantlets 2 weeks old (cv. Nipponbare). Treated and control (mock) rice leaves were collected 24 hours post inoculation. Three biological replicates for inoculated and control plants were extracted and analysed independently with the GeneChip® Rice Genome Array.
Publication: none
Created: 2012-04-26 10:24:51
Last Update: 2012-04-26 12:58:50
Released: 2012-05-01
GEO Accession GSE30942
Submitter: PLEXdb Curator
Name: Pamela Abbruscato
Institution: Parco Technologico Padano, Department of Rice Genomics Unit, Lodi, Italy
Head of Laboratory: Pamela Abbruscato
email(s):
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