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Gene expression in mature pollen and sperm cells versus young seedling as a vegetative sporophyte reference control
Scott D Russell, University of Oklahoma, Department of Botany and Microbiology, Norman, Oklahoma (srussell@ou.edu)
Experiment design (9 hybridizations)
developmental stage name
•seedling V3 stage •pollen at anthesis •sperm at anthesis

This experiment has been imported by PLEXdb from NCBI GEO (GSE17002)

Series_summary:
Sperm cells represent the male partner that fuse...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Gene expression in mature pollen and sperm cells versus young seedling as a vegetative sporophyte reference control
Accession No: OS94
Microarray: Rice57k
Visibility: public
Experiment Type:
Experiment Factor(s):
developmental stage name
•seedling V3 stage   •pollen at anthesis   •sperm at anthesis
Quality Control: biological replicates
Treatment summary:
 developmental stage name  # replicates
 seedling V3 stage  3
 pollen at anthesis  3
 sperm at anthesis  3
Total hybridizations: 9
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE17002)

Series_summary:
Sperm cells represent the male partner that fuses with the egg cell during fertilization in all multi-cellular eukaryotic organisms, and, in flowering plants, is a founder of both embryo and nutritive endosperm. We examined the transcriptome of Oryza sativa ssp. japonica using the Affymetrix 57K rice genome GeneChip to provide an overview of genes activated in the paternal gamete.
We used microarrays to detail the global program of gene expression in mature pollen and sperm cells at anthesis, which constitutes the stage immediately preceding pollen germination and gamete deposition leading up to fertilization. This documents the condition of the male gametophytic cells prior to fertilization.
Keywords: tissue expression profile, male gametophyte, sperm, pollen, seedling

Series_overall_design:
Male gametophytic cells consisting of isolated pollen and isolated sperm cells collected at anthesis were compared with seedlings containing leaf, stem, root and apical meristems as a vegetative sporophytic control; three separate biological replicates were used.
Publication: 'Transcriptome-based examination of putative pollen allergens of rice (Oryza sativa ssp. japonica).', Russell SD, Bhalla PL, Singh MB.
Mol Plant 2008 Sep;1(5):751-9.
pubmed: 19825578
Created: 2012-05-17 11:25:30
Last Update: 2012-05-17 15:04:22
Released: 2012-05-23
GEO Accession GSE17002
Submitter: PLEXdb Curator
Name: Scott D Russell
Institution: University of Oklahoma, Department of Botany and Microbiology, Norman, Oklahoma
Head of Laboratory: Scott D Russell
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