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Browse RicePLEX Experiment Data

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Genome-wide gene expression profiling of rice Zhonghua11 and ila1 lamina joint
Baocai Zhang, Institute of Genetics and Developmental Biology, CAS, Center for Genome Biology, Beijing, China (bczhang@genetics.ac.cn)
Experiment design (4 hybridizations)
genotype
•WT ZH11 •mutant ila1

This experiment has been imported by PLEXdb from NCBI GEO (GSE33361)

Series_summary:
The leaf lamina joint joins the rice leaf blade ...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Genome-wide gene expression profiling of rice Zhonghua11 and ila1 lamina joint
Accession No: OS95
Microarray: Rice57k
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•WT ZH11   •mutant ila1
Quality Control: biological replicates
Treatment summary:
 genotype  # replicates
 WT ZH11  2
 mutant ila1  2
Total hybridizations: 4
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE33361)

Series_summary:
The leaf lamina joint joins the rice leaf blade and sheath, contributing significantly to the leaf angle trait. A more erect leaf facilitates the penetration of sunlight, enhancing photosynthetic efficiency and occupying less space in dense planting. Genetic screening found a mutant increased leaf angle1, ila1 from rice T-DNA insertional mutants library.
We used microarrays to detail the transcriptional profile changes in the mutant ila1 lamina joint.

Series_overall_design:
Two biological replicate sample of leaf lamina joints from the ila1 and wild-type plants at tillering stage were collected for RNA extraction. Total RNAs were isolated from each replicate via the TRIzol method (Invitrogen) and used in target synthesis for the Rice Genome Array from Affymetrix. The microarray analyses were performed through following standard protocols (Affymetrix).
Publication: none
Created: 2012-06-26 10:09:12
Last Update: 2012-06-26 13:20:50
Released: 2012-06-28
GEO Accession GSE33361
Submitter: PLEXdb Curator
Name: Baocai Zhang
Institution: Institute of Genetics and Developmental Biology, CAS, Center for Genome Biology, Beijing, China
Head of Laboratory: --
email(s):
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