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Browse RicePLEX Experiment Data

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Nitrogen-efficient rice over-expressing alanine aminotransferase
Perrin Hudson Beatty, University of Alberta, Departement of Biological Sciences, Edmonton, Canada (pbeatty@ualberta.ca)
Experiment design (32 hybridizations)
organism part name
•shoot •root
genetic line
•wildtype-NB •AlaAT-ox-1/7 •AlaAT-ox-1/8 •AlaAT-ox-3/8

This experiment has been imported by PLEXdb from NCBI GEO (GSE39687)

Series_summary:
Oryza sativa cv. Nipponbare was engineered to ov...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Nitrogen-efficient rice over-expressing alanine aminotransferase
Accession No: OS98
Microarray: Rice57k
Visibility: public
Experiment Type:
Experiment Factor(s):
organism part name
•shoot   •root
genetic line
•wildtype-NB   •AlaAT-ox-1/7   •AlaAT-ox-1/8   •AlaAT-ox-3/8
Quality Control: biological replicates
Treatment summary:
 organism part name  genetic line  # replicates
 shoot  wildtype-NB  4
 shoot  AlaAT-ox-1/7  4
 shoot  AlaAT-ox-1/8  4
 shoot  AlaAT-ox-3/8  4
 root  wildtype-NB  4
 root  AlaAT-ox-1/7  4
 root  AlaAT-ox-1/8  4
 root  AlaAT-ox-3/8  4
Total hybridizations: 32
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE39687)

Series_summary:
Oryza sativa cv. Nipponbare was engineered to over-express a barley alanine aminotransferase (alaAT) gene using the promoter (OsANT1) from a rice aldehyde dehydrogenase gene that expresses in roots.
We are using biotechnology to improve the nitrogen use efficiency of rice by over-expressing alaAT in a tissue specific (root) manner. The AlaAT enzyme is a reversible aminotransferase that is linked to both C and N metabolism since it uses pyruvate plus glutamate to produce alanine and 2-oxoglutarate, and visa versa.

Series_overall_design:
Wildtype rice (Nipponbare) and three independent OsANT1:HvAlaAT rice transgenic lines (AGR1/7, AGR1/8 and AGR3/8) were grown hydroponically with 5mM NH4+ as the nitrogen source, to the reproductive stage. RNA samples were taken at active tillering, maximum tillering and end-of-tillering stages from root and shoot, at mid-day of the plants' day/night cycle. The RNA from root and shoot at maxiumum tillering was used for microarray analysis. Please read Beatty et al., 2009, Plant Biotechnology Journal 7, pp562-576 for further details..
Publication: 'Transcriptome analysis of nitrogen-efficient rice over-expressing alanine aminotransferase.', Beatty PH, Shrawat AK, Carroll RT, Zhu T, Good AG.
Plant Biotechnol J 2009 Aug;7(6):562-76.
pubmed: 19508275
Created: 2012-08-27 15:29:06
Last Update: 2012-09-10 17:34:57
Released: 2012-09-12
GEO Accession GSE39687
Submitter: PLEXdb Curator
Name: Perrin Hudson Beatty
Institution: University of Alberta, Departement of Biological Sciences, Edmonton, Canada
Head of Laboratory: Perrin Hudson Beatty
email(s):
Homepage:

 
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