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Experiment Name:

Nitrogen-efficient rice over-expressing alanine aminotransferase

Accession No:

OS98

Microarray:

Rice57k

Visibility:

public

Experiment Type:

Experiment Factor(s):

organism part name: •shoot •root
genetic line: •wildtype-NB •AlaAT-ox-1/7 •AlaAT-ox-1/8 •AlaAT-ox-3/8

Quality Control:

biological replicates

Treatment summary:

organism part name	genetic line	# replicates
shoot	wildtype-NB	4
shoot	AlaAT-ox-1/7	4
shoot	AlaAT-ox-1/8	4
shoot	AlaAT-ox-3/8	4
root	wildtype-NB	4
root	AlaAT-ox-1/7	4
root	AlaAT-ox-1/8	4
root	AlaAT-ox-3/8	4

Total hybridizations: 32

Description:

This experiment has been imported by PLEXdb from NCBI GEO (GSE39687)

Series_summary:
Oryza sativa cv. Nipponbare was engineered to over-express a barley alanine aminotransferase (alaAT) gene using the promoter (OsANT1) from a rice aldehyde dehydrogenase gene that expresses in roots.
We are using biotechnology to improve the nitrogen use efficiency of rice by over-expressing alaAT in a tissue specific (root) manner. The AlaAT enzyme is a reversible aminotransferase that is linked to both C and N metabolism since it uses pyruvate plus glutamate to produce alanine and 2-oxoglutarate, and visa versa.

Series_overall_design:
Wildtype rice (Nipponbare) and three independent OsANT1:HvAlaAT rice transgenic lines (AGR1/7, AGR1/8 and AGR3/8) were grown hydroponically with 5mM NH4+ as the nitrogen source, to the reproductive stage. RNA samples were taken at active tillering, maximum tillering and end-of-tillering stages from root and shoot, at mid-day of the plants' day/night cycle. The RNA from root and shoot at maxiumum tillering was used for microarray analysis. Please read Beatty et al., 2009, Plant Biotechnology Journal 7, pp562-576 for further details..

Publication:

'Transcriptome analysis of nitrogen-efficient rice over-expressing alanine aminotransferase.', Beatty PH, Shrawat AK, Carroll RT, Zhu T, Good AG.
Plant Biotechnol J 2009 Aug;7(6):562-76.
pubmed: 19508275

Created:

2012-08-27 15:29:06

Last Update:

2012-09-10 17:34:57

Released:

2012-09-12

GEO Accession

GSE39687

Submitter:

PLEXdb Curator

Experimenter:

Name:	Perrin Hudson Beatty
Institution:	University of Alberta, Departement of Biological Sciences, Edmonton, Canada
Head of Laboratory:	Perrin Hudson Beatty
email(s):	pbeatty@ualberta.ca,pbeatty@ualberta.ca
Homepage:


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