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Comparative transcriptomic reveals signatures specific to various interactions with Melampsora rust fungi
Armand,,Séguin, Laurentian forestry Service, Québec, Canada (Armand.Seguin@rncan.gc.ca)
Experiment design (12 hybridizations)
infection
•Non_infected •Mlp_infected •Mmd_infected •Mixed_infected

This experiment has been imported by PLEXdb from NCBI GEO (GSE9673)

Series_summary: We aim to understand the global transcriptional res...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Comparative transcriptomic reveals signatures specific to various interactions with Melampsora rust fungi
Accession No: PT1
Microarray: Poplar61k
Visibility: public
Experiment Type:
Experiment Factor(s):
infection
•Non_infected   •Mlp_infected   •Mmd_infected   •Mixed_infected
Quality Control: biological replicates
Treatment summary:
 infection  # replicates
 Non_infected  3
 Mlp_infected  3
 Mmd_infected  3
 Mixed_infected  3
Total hybridizations: 12
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE9673)

Series_summary:
We aim to understand the global transcriptional response of hybrid poplar NM6 (Populus nigra x Populus maximoviczii) to infection by two biotrophic Melampsora fungi, M. larici-populina and M. medusae f. sp. deltoidae separately and simultanously. Transcript profilling revealed 699 differentially expressed genes whose expression level was equal or higher than 2-fold between infected and noninfected tissues. In the mixed infection, our results showed an additive response of the two pathogens.
Genes regulated as a result of Melampsora infection were generally induced and mainly involved in primary/secondary metabolism that include cell wall reinforcement and lignification, defense-related processes like pathogenesis-related and stress response, signal perception and transduction mechanisms and regulation of transcription.

Keywords: Stress response

Series_overall_design:
Poplar leaves were infected with Melampsora larici-populina and Melampsora medusae f. sp. deltoidae and the mixture of them (Mixed infection). At six day post-infection, tissues were harvested for RNA extraction and hybridization on Affymetrix microarrays. Three biological replications were obtained from infected and non infected leaves (control samples).
Publication: 'Transcriptome profiling in hybrid poplar following interactions with Melampsora rust fungi', Azaiez A, Boyle B, Levée V, Séguin A
Mol Plant Microbe Interact 2009 Feb;22(2):190-200
pubmed: 19132871
Created: 2009-07-06 15:27:28
Last Update: 2009-07-06 15:38:50
GEO Accession GSE9673
Submitter: PLEXdb Curator
Name: Armand,,Séguin
Institution: Laurentian forestry Service, Québec, Canada
Head of Laboratory: --
email(s):
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