Search   for 
Find Your Gene ·  Publications ·  Tools ·  Gene List Suite ·  Expression Atlases ·  About PLEXdb ·  Feedback
Login ID:
Password:
Plants
Arabidopsis
Barley
Brachypodium
Citrus
Cotton
Grape
Maize
Medicago
Poplar
Rice
Soybean
Sugarcane
Tomato
Wheat
Pathogens & Symbionts
Search Experiments
Download Data
Gene List Suite
Tools
Your Account
Documentation
News
Personnel
Links
 Data Release Policy 
PLEXdb is committed to making expression data publicly available to researchers world-wide...
complete policy
 

Browse PoplarPLEX Experiment Data

Choose an experiment:
Habituation to thaxtomin A provides enhanced and durable resistance to inhibitors of cellulose synthesis
Nathalie Beaudoin, Universite de Sherbrooke, Faculte des Sciences, Departement de Biologie, 2500, Blvd Universite, Sherbrooke, Quebec, J1K 2R1, Canada (--)
Experiment design (6 hybridizations)
compound
•Non_Habituated •TA_Habituated

This experiment has been imported by PLEXdb from NCBI GEO (GSE17804)

Series_summary: TA inhibits cellulose synthesis but its actual mode o...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

Show
Experiment Name: Habituation to thaxtomin A provides enhanced and durable resistance to inhibitors of cellulose synthesis
Accession No: PT10
Microarray: Poplar61k
Visibility: public
Experiment Type:
Experiment Factor(s):
compound
•Non_Habituated   •TA_Habituated
Quality Control:
Treatment summary:
 compound  # replicates
 Non_Habituated  3
 TA_Habituated  3
Total hybridizations: 6
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE17804)

Series_summary: TA inhibits cellulose synthesis but its actual mode of action is unknown. Addition of TA to hybrid poplar (Populus trichocarpa x Populus deltoides) cell suspensions can activate a cellular program leading to cell death. In contrast, it is possible to habituate hybrid poplar cell cultures to grow in the presence of TA levels that would normally induce cell death. This habituation was performed by adding increasing levels of TA to cell cultures at the time of subculture over a period of 12 months. TA-habituated cells were then cultured in the absence of TA for more than 18 months.
These cells displayed a reduced size and growth compared to control cells and had fragmented vacuoles filled with electron-dense material. Habituation to TA was associated with changes in the cell wall composition, with a reduction in cellulose and an increase in pectin levels. Remarkably, high level of resistance to TA was maintained in TA-habituated cells even after being cultured in the absence of TA. Moreover, these cells exhibited enhanced resistance to two other inhibitors of cellulose biosynthesis, dichlobenil and isoxaben. Analysis of gene expression in TA-habituated cells using Affymetrix GeneChip Poplar Genome Array revealed that durable resistance to TA is associated with a major and complex reprogramming of gene expression implicating processes such as cell wall synthesis and modification, lignin and flavonoid synthesis, as well as DNA and chromatin modifications.

Series_overall_design:
Each sample was taken from an individual flask of control cells or TA-habituated resistant cells grown without the toxin for 18 months (TA(-)hab cells) that had grown for 5 d after subculture. Six arrays were hybridized, representing 3 arrays per cell type.
Publication: none
Created: 2010-04-09 13:30:55
Last Update: 2010-07-02 13:51:46
Released: 2010-06-30
GEO Accession GSE17804
Submitter: PLEXdb Curator
Name: Nathalie Beaudoin
Institution: Universite de Sherbrooke, Faculte des Sciences, Departement de Biologie, 2500, Blvd Universite, Sherbrooke, Quebec, J1K 2R1, Canada
Head of Laboratory: --
email(s):
Homepage:

 
Copyright@2001-2013 The PLEXdb Group
All rights reserved.

For problems with the webpages contact PLEXdb webmasters