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Browse PoplarPLEX Experiment Data

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Expression data from poplar apices
Victor Busov, Michigan Technological University, Department of Forest Resources and Environmental Science, Houghton, MI (vbusov@mtu.edu)
Experiment design (4 hybridizations)
genotype
•P35S::EBB1 •wildtype

This experiment has been imported by PLEXdb from NCBI GEO (GSE16495) Series_summary: We studied differences in gene expression between Populus...[complete overview]

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Experiment Name: Expression data from poplar apices
Accession No: PT13
Microarray: Poplar61k
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•P35S::EBB1   •wildtype
Quality Control: biological replicates
Treatment summary:
 genotype  # replicates
 P35S::EBB1  2
 wildtype  2
Total hybridizations: 4
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE16495)

Series_summary:
We studied differences in gene expression between Populus P35S::EBB1 lines and control, affecting plant growth and differentiation, and dormancy. We used microarrays to detail the global program of gene expression underlying morphological and developmental changes driven by overexpression of the EBB1 gene.
By screening activation tagging population, we identified the EARLY BUD-BREAK1 (EBB1) mutant. We positioned the tag, localized a putative candidate gene and verified transcription activation. The activated gene encodes an AP2/ERF transcription factor similar to a small B1 gene-subfamily in Arabidopsis encoding strong regulators of meristem function and lateral organ outgrowth. We fully recapitulated the phenotype by overexpression of the gene into the same genotype under a strong constitutive promoter (P35S::EBB1). We also found that EBB1 transcript abundance in wild type plants increases during the period prior to bud-break, and in response to the hormone cytokinin. Our data indicates that EBB1 plays an important role in the process of bud-break.

Series_overall_design:
Poplar apex was selected for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain homogeneous native expression of affected genes in P35S::EBB1 lines and control, in order to increase the resolution of expression profiles inducing the developmental changes in P35S::EBB1. To do that, we selected apex tissue from greenhouse healthy plants.
Publication: none
Created: 2010-12-06 14:13:48
Last Update: 2010-12-06 14:27:50
Released: 2010-12-08
GEO Accession GSE16495
Submitter: PLEXdb Curator
Name: Victor Busov
Institution: Michigan Technological University, Department of Forest Resources and Environmental Science, Houghton, MI
Head of Laboratory: Victor Busov
email(s):
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