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Genotype and time of day shape the Populus drought response
Malcolm M. Campbell, University of Toronto, Department of Cell & Systems Biology, Toronto, Canada (malcolm.campbell@utoronto.ca)
Experiment design (48 hybridizations)
genotype
•DN34 •NM6
time
•midnight •predawn •midday •lateday
stress condition
•well watered •water limited

This experiment has been imported by PLEXdb from NCBI GEO (GSE15242)

Series_summary:
As exposure to episodic drought can impinge sign...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Genotype and time of day shape the Populus drought response
Accession No: PT22
Microarray: Poplar61k
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•DN34   •NM6
time
•midnight   •predawn   •midday   •lateday
stress condition
•well watered   •water limited
Quality Control: biological replicates
Treatment summary:
 genotype  time  stress condition  # replicates
 DN34  midnight  well watered  3
 DN34  midnight  water limited  3
 DN34  predawn  well watered  3
 DN34  predawn  water limited  3
 DN34  midday  well watered  3
 DN34  midday  water limited  3
 DN34  lateday  well watered  3
 DN34  lateday  water limited  3
 NM6  midnight  well watered  3
 NM6  midnight  water limited  3
 NM6  predawn  well watered  3
 NM6  predawn  water limited  3
 NM6  midday  well watered  3
 NM6  midday  water limited  3
 NM6  lateday  well watered  3
 NM6  lateday  water limited  3
Total hybridizations: 48
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE15242)

Series_summary:
As exposure to episodic drought can impinge significantly on forest health and the establishment of productive tree plantations, there is great interest in understanding the mechanisms of drought response in trees. The ecologically dominant and economically important genus Populus, with its sequenced genome, provides an ideal opportunity to examine transcriptome level changes in trees in response to a drought stimulus. The transcriptome level drought response of two commercially important hybrid Populus clones (P. deltoides · P. nigra, DN34, and P. nigra · P. maximowiczii, NM6) was characterized over a diurnal period using a 4 · 2 · 2 completely randomized factorial ANOVA experimental design (four time points, two genotypes, and two treatment conditions) using Affymetrix Poplar GeneChip microarrays. Notably, the specific genes that exhibited changes in transcript abundance in response to drought differed between the genotypes and/or the time of day that they exhibited their greatest differences. This study emphasizes the fact that it is not possible to draw simple, generalized conclusions about the drought response of the genus Populus on the basis of one species, nor on the basis of results collected at a single time point. The data derived from our studies provide insights into the variety of genetic mechanisms underpinning the Populus drought response, and provide candidates for future experiments aimed at understanding this response across this economically and ecologically important genus.

Series_overall_design:
48 arrays total. 2 genotypes (DN34, NM6), 4 time points (midnight, pre-dawn, mid-day, late day), 2 water regimes (well-watered, water-limited). 3 biological replicates per treatment.
Publication: 'Genotype and time of day shape the Populus drought response.', Wilkins O, Waldron L, Nahal H, Provart NJ, Campbell MM.
Plant J 2009 Nov;60(4):703-15.
pubmed: 19682285
Created: 2011-06-27 14:04:35
Last Update: 2011-06-29 12:04:15
Released: 2011-06-29
GEO Accession GSE15242
Submitter: PLEXdb Curator
Name: Malcolm M. Campbell
Institution: University of Toronto, Department of Cell & Systems Biology, Toronto, Canada
Head of Laboratory: Malcolm M. Campbell
email(s):
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