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Browse PoplarPLEX Experiment Data

Choose an experiment:
Poplar cultivars Soligo and Carpacio
Sandrine Balzergue, INRA, Department of URGV, Evry, France (balzerg@evry.inra.fr)
Experiment design (4 hybridizations)
genetic line
•Soligo gDNA •Carpacio gDNA

This experiment has been imported by PLEXdb from NCBI GEO (GSE21334)

Series_summary:
affy_genomic_poplar - affy_genomic_poplar - The...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Poplar cultivars Soligo and Carpacio
Accession No: PT24
Microarray: Poplar61k
Visibility: public
Experiment Type:
Experiment Factor(s):
genetic line
•Soligo gDNA   •Carpacio gDNA
Quality Control: biological replicates
Treatment summary:
 genetic line  # replicates
 Soligo gDNA  2
 Carpacio gDNA  2
Total hybridizations: 4
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE21334)

Series_summary:
affy_genomic_poplar - affy_genomic_poplar - The project aims to identify genes of interest for water deficit acclimation in poplar. We look for genes and gene expression networks related to drought stress in two hybrid cultivars, differing in their drought tolerance in field. Affymetrix poplar genome array was designed on several Populus species. In order to deal with comparative approaches, we checked the convenience of the array by hybridizing genomic DNA of the two hybrid cultivars (Populus deltoides × Populus nigra, namely ‘cv Carpaccio’ and ‘cv Soligo’). This point is important as transcript sequence might have diverged in the two genomes (Fossati et al, 2005), which could lead to absence of hybridization without physiological meaning. -Two poplar cultivars, Soligo (S) and Carpacio (C) were grown in controlled conditions. Mature leaves were collected and genomic DNA was extracted from leaves in CTAB buffer. gDNA was fragmented with DNAse1. DNA fragments were labelled with Biotin N6-ddATP and hybridized on Affymetrix poplar genome array. Two technical replicates per genotype were performed.

Keywords: genomic comparison,gain of fuction epimutation

Series_overall_design:
4 arrays - poplar
Publication: none
Created: 2011-07-25 12:40:04
Last Update: 2011-07-25 16:14:18
Released: 2011-07-25
GEO Accession GSE21334
Submitter: PLEXdb Curator
Name: Sandrine Balzergue
Institution: INRA, Department of URGV, Evry, France
Head of Laboratory: Claire Lurin
email(s):
Homepage:

 
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