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Browse PoplarPLEX Experiment Data

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affy_popsec_nancy_pophydro_roots_poplar -Molecular bases of acclimation and adaptation to water deficit in poplar
Sandrine Balzergue, INRA, Department of URGV, Evry, France (balzerg@evry.inra.fr)
Experiment design (4 hybridizations)
stress condition
•control •water deficit(PEG)

This experiment has been imported by PLEXdb from NCBI GEO (GSE17225)

Series_summary:
affy_popsec_nancy_pophydro_roots_poplar - This ...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: affy_popsec_nancy_pophydro_roots_poplar -Molecular bases of acclimation and adaptation to water deficit in poplar
Accession No: PT25
Microarray: Poplar61k
Visibility: public
Experiment Type:
Experiment Factor(s):
stress condition
•control   •water deficit(PEG)
Quality Control: biological replicates
Treatment summary:
 stress condition  # replicates
 control  2
 water deficit(PEG)  2
Total hybridizations: 4
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE17225)

Series_summary:
affy_popsec_nancy_pophydro_roots_poplar - This project aims to identify genes of interest for control of root growth in response to water deficit in a tree species: poplar. We look for genes and gene expression networks related to drought stress. We intend to analyse the transcriptome in root apices of cuttings grown in hydroponics and osmotically stressed with PEG. Root apex is the location of root elongation and these analyses intend to identify genes involved in the control of cell expansion and thus of root elongation. Indeed, root growth maintenance in response to water shortage contributes to plant tolerance to water deficit.-Poplar cuttings (cv Soligo) were grown in hydroponics. A moderate water deficit was applied by adding PEG to the nutrient solution (200g/l). After 3 days, the apex (1 cm-long) of the roots of each cutting were collected. For control and stressed treatments, RNAs were extracted from two pools of 5 to 9 roots (issued from 2 to 5 cuttings). A pool is considered as one biological replicate and corresponds to one Affymetrix slide.

Keywords: treated vs untreated comparison

Series_overall_design:
4 arrays - poplar
Publication: none
Created: 2011-07-25 12:58:57
Last Update: 2011-07-25 16:24:10
Released: 2011-07-25
GEO Accession GSE17225
Submitter: PLEXdb Curator
Name: Sandrine Balzergue
Institution: INRA, Department of URGV, Evry, France
Head of Laboratory: Claire Lurin
email(s):
Homepage:

 
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