Search   for 
Find Your Gene ·  Publications ·  Tools ·  Gene List Suite ·  Expression Atlases ·  About PLEXdb ·  Feedback
Login ID:
Password:
Plants
Arabidopsis
Barley
Brachypodium
Citrus
Cotton
Grape
Maize
Medicago
Poplar
Rice
Soybean
Sugarcane
Tomato
Wheat
Pathogens & Symbionts
Search Experiments
Download Data
Gene List Suite
Tools
Your Account
Documentation
News
Personnel
Links
 Data Release Policy 
PLEXdb is committed to making expression data publicly available to researchers world-wide...
complete policy
 

Browse PoplarPLEX Experiment Data

Choose an experiment:
affy_popsec_nancy_roots_poplar -Molecular bases of acclimation and adaptation to water deficit in poplar
Sandrine Balzergue, INRA, Department of URGV, Evry, France (balzerg@evry.inra.fr)
Experiment design (16 hybridizations)
cultivar
•Soligo •Carpacio
stress condition
•Control(CTL) •mild water deficit(AMI) •moderate water deficit(AMO) •early-drought stress(EAR)

This experiment has been imported by PLEXdb from NCBI GEO (GSE17223)

Series_summary:
affy_popsec_nancy_roots_poplar - This project ai...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

Show
Experiment Name: affy_popsec_nancy_roots_poplar -Molecular bases of acclimation and adaptation to water deficit in poplar
Accession No: PT26
Microarray: Poplar61k
Visibility: public
Experiment Type:
Experiment Factor(s):
cultivar
•Soligo   •Carpacio
stress condition
•Control(CTL)   •mild water deficit(AMI)   •moderate water deficit(AMO)   •early-drought stress(EAR)
Quality Control: biological replicates
Treatment summary:
 cultivar  stress condition  # replicates
 Soligo  Control(CTL)  2
 Soligo  mild water deficit(AMI)  2
 Soligo  moderate water deficit(AMO)  2
 Soligo  early-drought stress(EAR)  2
 Carpacio  Control(CTL)  2
 Carpacio  mild water deficit(AMI)  2
 Carpacio  moderate water deficit(AMO)  2
 Carpacio  early-drought stress(EAR)  2
Total hybridizations: 16
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE17223)

Series_summary:
affy_popsec_nancy_roots_poplar - This project aims to identify genes of interest for water deficit acclimation and/or adaptation in a tree species: poplar. We look for genes and gene expression networks related to drought stress. We intend to analyse the transcriptome in root apices, in two genotypes, Carpaccio and Soligo, at various stages and intensities of stress. Root apex is the location of root elongation and these analyses intend to identify genes involved in the control of cell expansion and thus of root elongation. Indeed, root growth maintenance in response to water shortage contributes to plant tolerance to water deficit. The comparison between medium and severe stress intensities and between early and long term stresses will power the selection of genes of interest. The co-analysis of two genotypes of contrasted tolerance to water deficit should help to better discriminate genes presenting a potential adaptative character from genes responding passively to the constraint.-Two poplar clones, Soligo (S) and Carpacio (C) were submitted to 4 treatments: control, mild water deficit, moderate water deficit (12-day long for both) and early-drought stress (about 36-h long). Growth and physiology was characterised on a batch of plants and samples collected on another batch of plants. Four to eight root apices (1 cm-long) were collected on each individual tree. Total RNAs were extracted from all roots for each tree individually. Two pools of 3 (or 2) individuals were made using equimolar ratio. A pool is considered as one biological replicate and corresponds to one Affymetrix slide. The two biological replicates originate from the same experiment.

Keywords: treated vs untreated comparison

Series_overall_design:
16 arrays - poplar
Publication: none
Created: 2011-07-25 13:15:42
Last Update: 2011-07-27 14:30:42
Released: 2011-07-27
GEO Accession GSE17223
Submitter: PLEXdb Curator
Name: Sandrine Balzergue
Institution: INRA, Department of URGV, Evry, France
Head of Laboratory: Claire Lurin
email(s):
Homepage:

 
Copyright@2001-2013 The PLEXdb Group
All rights reserved.

For problems with the webpages contact PLEXdb webmasters