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Browse PoplarPLEX Experiment Data

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affy_popsec_nancy_leaves2007_poplar -Molecular bases of acclimatation and adaptation to water deficit in poplar
Sandrine Balzergue, INRA, Department of URGV, Evry, France (balzerg@evry.inra.fr)
Experiment design (16 hybridizations)
genotype
•Soligo •Carpacio
stress condition
•control •mild water deficit •moderate water deficit •early-drought stress

This experiment has been imported by PLEXdb from NCBI GEO (GSE17230)

Series_summary:
affy_popsec_nancy_leaves_poplar - affy_popsec_n...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: affy_popsec_nancy_leaves2007_poplar -Molecular bases of acclimatation and adaptation to water deficit in poplar
Accession No: PT28
Microarray: Poplar61k
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•Soligo   •Carpacio
stress condition
•control   •mild water deficit   •moderate water deficit   •early-drought stress
Quality Control: biological replicates
Treatment summary:
 genotype  stress condition  # replicates
 Soligo  control  2
 Soligo  mild water deficit  2
 Soligo  moderate water deficit  2
 Soligo  early-drought stress  2
 Carpacio  control  2
 Carpacio  mild water deficit  2
 Carpacio  moderate water deficit  2
 Carpacio  early-drought stress  2
Total hybridizations: 16
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE17230)

Series_summary:
affy_popsec_nancy_leaves_poplar - affy_popsec_nancy_leaves2007_poplar - This project aims to identify genes of interest for water deficit acclimation and/or adaptation in a tree species: poplar. We look for genes and gene expression networks related to drought stress. We intend to analyse the transcriptome in mature leaves, in two genotypes, Carpaccio and Soligo, at various stages and intensities of stress. During water deficit, leaves underwent many processes aiming to maintain cells integrity such as water status adjustment through osmoregulation or cell detoxication. These analyses intend to identify genes of interest involved in homeostasis maintenance. The comparison between medium and severe stress intensities and between early and long term stresses will power the selection of genes of interest. The co-analysis of two genotypes of contrasted tolerance to water deficit should help to discriminate genes presenting a potential adaptative character from genes responding passively to the constraint-In a first experiment, two poplar clones, Soligo (S) and Carpacio (C) were submitted to 4 treatments: control, mild water deficit, moderate water deficit (12-day long for both) and early-drought stress (about 36-h long). Growth and physiology was characterised on a batch of plants and samples collected on another batch of plants. In a second experiment, two poplar clones, Soligo (S) and Carpacio (C) were submitted to 2 treatments: control and moderate water deficit. Mature leaves were collected and total RNAs were extracted from each tree individually. Two pools of 3 (or 2) individuals were made using equimolar ratio. A pool is considered as one biological replicate and corresponds to one Affimetrix slide.

Keywords: treated vs untreated comparison

Series_overall_design:
16 arrays - poplar
Publication: none
Created: 2011-07-27 11:57:42
Last Update: 2011-07-27 15:14:09
Released: 2011-07-27
GEO Accession GSE17230
Submitter: PLEXdb Curator
Name: Sandrine Balzergue
Institution: INRA, Department of URGV, Evry, France
Head of Laboratory: Claire Lurin
email(s):
Homepage:

 
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