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Browse PoplarPLEX Experiment Data

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Clone History Shapes Populus Drought Responses
Malcolm M. Campbell, University of Toronto, Department of Cell & Systems Biology, Toronto, Canada (malcolm.campbell@utoronto.ca)
Experiment design (72 hybridizations)
time
•A.M. •P.M.
genetic line
•DN(MB) •DN(SK) •WK(AB) •WK(SK) •WP(AB) •WP(SK)
growth condition
•well-watered •water-limited

This experiment has been imported by PLEXdb from NCBI GEO (GSE27693)

Series_summary:
Just as animal monozygotic twins can experience ...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Clone History Shapes Populus Drought Responses
Accession No: PT33
Microarray: Poplar61k
Visibility: public
Experiment Type:
Experiment Factor(s):
time
•A.M.   •P.M.
genetic line
•DN(MB)   •DN(SK)   •WK(AB)   •WK(SK)   •WP(AB)   •WP(SK)
growth condition
•well-watered   •water-limited
Quality Control: biological replicates
Treatment summary:
 time  genetic line  growth condition  # replicates
 A.M.  DN(MB)  well-watered  3
 A.M.  DN(MB)  water-limited  3
 A.M.  DN(SK)  well-watered  3
 A.M.  DN(SK)  water-limited  3
 A.M.  WK(AB)  well-watered  3
 A.M.  WK(AB)  water-limited  3
 A.M.  WK(SK)  well-watered  3
 A.M.  WK(SK)  water-limited  3
 A.M.  WP(AB)  well-watered  3
 A.M.  WP(AB)  water-limited  3
 A.M.  WP(SK)  well-watered  3
 A.M.  WP(SK)  water-limited  3
 P.M.  DN(MB)  well-watered  3
 P.M.  DN(MB)  water-limited  3
 P.M.  DN(SK)  well-watered  3
 P.M.  DN(SK)  water-limited  3
 P.M.  WK(AB)  well-watered  3
 P.M.  WK(AB)  water-limited  3
 P.M.  WK(SK)  well-watered  3
 P.M.  WK(SK)  water-limited  3
 P.M.  WP(AB)  well-watered  3
 P.M.  WP(AB)  water-limited  3
 P.M.  WP(SK)  well-watered  3
 P.M.  WP(SK)  water-limited  3
Total hybridizations: 72
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE27693)

Series_summary:
Just as animal monozygotic twins can experience different environmental conditions by being reared apart, individual genetically-identical trees of the genus Populus can also be exposed to contrasting environmental conditions by being grown in different locations. As such, clonally-propagated Populus trees provide an opportunity to interrogate the impact of individual environmental history on current response to environmental stimuli. To test the hypothesis that current responses to an environmental stimulus, drought, are contingent on environmental history, the transcriptome-level drought responses of three economically important hybrid genotypes: DN34 (Populus deltoides x P. nigra); Walker (P. deltoides var. occidentalis x (P. laurifolia x P. nigra)); and, Okanese (‘Walker’ x (P. laurifolia x P. nigra)) derived from two different locations were compared. Strikingly, differences in transcript abundance patterns in response to drought were based on differences in geographic origin of clones for two of the three genotypes. This observation was most pronounced for the genotypes with the longest time since establishment and last common propagation. Differences in genome-wide DNA methylation paralleled the transcriptome level trends, where the clones with the most divergent transcriptomes and clone history had the most marked differences in the extent of total DNA methylation, suggesting an epigenetic basis for the clone-history-dependent transcriptome divergence. The data provide insights into the interplay between genotype and environment in the ecologically and economically important Populus genus, with implications for both the industrial application of Populus trees, and the evolution and persistence of these important tree species.

Series_overall_design:
72 arrays total. 2 time points. 2 water regimes. 3 biological replicates per treatment
Publication: none
Created: 2011-07-29 10:38:31
Last Update: 2011-08-03 16:52:46
Released: 2011-08-03
GEO Accession GSE27693
Submitter: PLEXdb Curator
Name: Malcolm M. Campbell
Institution: University of Toronto, Department of Cell & Systems Biology, Toronto, Canada
Head of Laboratory: Malcolm M. Campbell
email(s):
Homepage:

 
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