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Browse PoplarPLEX Experiment Data

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affy_popsec_nancy_stomata_poplar -Molecular bases of acclimation and adaptation to water deficit in poplar
Sandrine Balzergue, INRA, Department of URGV, Evry, France (balzerg@evry.inra.fr)
Experiment design (16 hybridizations)
genotype
•Carpaccio •Soligo
water stress
•control •early-drought stress •mild water deficit •moderate water deficit

This experiment has been imported by PLEXdb from NCBI GEO (GSE17219)

Series_summary:
affy_popsec_nancy_stomata_poplar - This project a...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: affy_popsec_nancy_stomata_poplar -Molecular bases of acclimation and adaptation to water deficit in poplar
Accession No: PT44
Microarray: Poplar61k
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•Carpaccio   •Soligo
water stress
•control   •early-drought stress   •mild water deficit   •moderate water deficit
Quality Control: biological replicates
Treatment summary:
 genotype  water stress  # replicates
 Carpaccio  control  2
 Carpaccio  early-drought stress  2
 Carpaccio  mild water deficit  2
 Carpaccio  moderate water deficit  2
 Soligo  control  2
 Soligo  early-drought stress  2
 Soligo  mild water deficit  2
 Soligo  moderate water deficit  2
Total hybridizations: 16
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE17219)

Series_summary:
affy_popsec_nancy_stomata_poplar - This project aims to identify genes of interest for water deficit acclimation and/or adaptation in a tree species: poplar. We look for genes and gene expression networks related to drought stress. We intend to analyse the transcriptome in laser-microdissected stomata, in two genotypes, Carpaccio and Soligo, at various stages and intensities of stress. In response to water deficit, water loss is adjusted through stomatal conductance. Transcriptome of pure stomatal material should bring valuable information hardly reachable in whole leaf transcriptome analysis. The comparison between medium and severe stress intensities and between early and long term stresses will power the selection of genes of interest. The co-analysis of two genotypes of contrasted tolerance to water deficit should help to discriminate genes presenting a potential adaptative character from genes responding passively to the constraint.-Two poplar clones, Soligo (S) and Carpaccio (C) were submitted to 4 treatments: control, mild water deficit, moderate water deficit (12-day long for both) and early-drought stress (about 36-h long). Growth and physiology was characterised on a batch of plants and samples collected on another batch of plants. Five hundred stomata (one thousand five hundreds guard cells) were laser microdissected in mature leaves of each tree. Stomata from 3 trees (1500) were pooled for total RNAs extraction. A pool is considered as one biological replicate and corresponds to one Affymetrix slide. The two biological replicates originate from the same experiment.
Keywords: treated vs untreated comparison

Series_overall_design:
16 arrays - poplar

PLEXdb Curator Notes:
Key to GEO Samples:
SCTLS = Soligo/control
CEARS = Carpaccio/early-drought stress
CAMIS = Carpaccio/mild water deficit
SAMOS = Soligo/moderate water deficit
CCTLS = Carpaccio/control
CAMOS = Carpaccio/moderate water deficit
SEARS = Soligo/early-drought stress
SAMIS = Soligo/mild water deficit
Publication: none
Created: 2012-08-22 15:04:33
Last Update: 2012-08-29 15:18:29
Released: 2012-09-10
GEO Accession GSE17219
Submitter: PLEXdb Curator
Name: Sandrine Balzergue
Institution: INRA, Department of URGV, Evry, France
Head of Laboratory: Claire Lurin
email(s):
Homepage:

 
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