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Transcriptome analysis of a wheat NIL pair carrying FHB resistant and susceptible alleles
Seungho Cho, BASF Plant Science, LLC (seungho.cho@basf.com,muehl003@umn.edu)
Experiment design (24 hybridizations)
genotype
•resistant •susceptible
treated or untreated
•Fusarium graminearum strain Butte 86 •water
time
•48 hai •96 hai

In this study, we used the Affymetrix wheat GeneChip to examine the transcript accumulation in a near-isogenic line pair carrying resistant and sus...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Transcriptome analysis of a wheat NIL pair carrying FHB resistant and susceptible alleles
Accession No: TA20
Microarray: Wheat
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•resistant   •susceptible
treated or untreated
•Fusarium graminearum strain Butte 86   •water
time
•48 hai  •96 hai
Quality Control: biological replicates
Treatment summary:
 genotype  treated or untreated  time  # replicates
 resistant  Fusarium graminearum strain Butte 86  48 hai  3
 resistant  Fusarium graminearum strain Butte 86  96 hai  3
 resistant  water  48 hai  3
 resistant  water  96 hai  3
 susceptible  Fusarium graminearum strain Butte 86  48 hai  3
 susceptible  Fusarium graminearum strain Butte 86  96 hai  3
 susceptible  water  48 hai  3
 susceptible  water  96 hai  3
Total hybridizations: 24
Description: In this study, we used the Affymetrix wheat GeneChip to examine the transcript accumulation in a near-isogenic line pair carrying resistant and susceptible alleles at the wheat Fhb1 locus. The objectives of this study were: (1) to identify the overall response in wheat to F. graminearum infection; (2) to identify key genes involved in FHB resistance/susceptibility pathways in wheat; (3) to compare the transcript profiles of wheat and barley during F. graminearum infection; and (4) to examine the relationship between transcript accumulation, disease severity, fungal biomass and trichothecene accumulation in wheat.

Submitted at NCBI GEO via PLEXdb.



Submitted at NCBI GEO via PLEXdb.

Publication: 'Transcriptome Analysis of a Wheat Near-Isogenic Line Pair Carrying Fusarium Head Blight-Resistant and -Susceptible Alleles', Haiyan Jia, Seungho Cho, Gary J. Muehlbauer
Molecular Plant-Microbe Interactions 22(11):1366-1378 (2009)
Created: 2008-04-01 14:14:29
Last Update: 2008-05-07 13:14:56
Released: 2009-12-11
GEO Accession GSE31758
Submitter: Haiyan Jia
Name: Seungho Cho
Institution: BASF Plant Science, LLC
Head of Laboratory: Gary Muehlbauer
email(s):
Homepage:

 
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