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Transcription profiling wheat responses to adapted and non-adapted isolates of the blast fungus, Magnaporthe
Graham McGrann, John Innes Centre (graham.mcgrann@bbsrc.ac.uk, lesley.boyd@bbsrc.ac.uk)
Experiment design (12 hybridizations)
pathogen isolates
•Mock-inoculated (Control) •Wheat non-adapted Magnaporthe isolate BR29 •Wheat adapted Magnaporthe isolate BR32 •Wheat adapted Magnaporthe isolate BR37

Transcriptional changes were monitored in the wheat cultivar Renan 24 hours post i noculation with adapted and non-adapted Magnaporthe isolates usi...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Transcription profiling wheat responses to adapted and non-adapted isolates of the blast fungus, Magnaporthe
Accession No: TA24
Microarray: Wheat
Visibility: public
Experiment Type:
Experiment Factor(s):
pathogen isolates
•Mock-inoculated (Control)   •Wheat non-adapted Magnaporthe isolate BR29   •Wheat adapted Magnaporthe isolate BR32   •Wheat adapted Magnaporthe isolate BR37
Quality Control: biological replicates
Treatment summary:
 pathogen isolates  # replicates
 Mock-inoculated (Control)  3
 Wheat non-adapted Magnaporthe isolate BR29  3
 Wheat adapted Magnaporthe isolate BR32  3
 Wheat adapted Magnaporthe isolate BR37  3
Total hybridizations: 12
Description: Transcriptional changes were monitored in the wheat cultivar Renan 24 hours post i noculation with adapted and non-adapted Magnaporthe isolates using the Affymetrix wheat genome array GeneChip®. Wheat plants cv. Renan were grown in a peat and sand (1:1) mix at 23 ˚C in a Sanyo Fitotron growth cabinet (Sanyo Gallenkamp PLC, Loughborough, U.K.) with a 16/8 h, light/dark cycle. Three Magnaporthe isolates were used in this expt, two wheat-adapted isolates (BR32, BR37) and one wheat non-adapted isolate (BR29). Magnaporthe isolates were grown for eleven days on Complete Media Agar at 25C under a 16/8h, light/dark cycle. Conidia were harvested by flooding the plates with 5 mL of sterile inoculation solution [0.25% (w/v) gelatine and 0.01% (v/v) Tween 20] and scraping the conidia from the surface using a sterile glass rod. Conidia were filtered through sterile miracloth and the density adjusted to 1 x 10 5 conidia mL-1 with inoculation solution.
Fourteen day old wheat seedlings mist inoculated with 4 mL of a Magnaporthe conidia suspension and plants were sealed in plastic propagators to maintain relative humidity c.100% and kept at 25 ˚C in the dark for the first 24 hours post inoculation (hpi). Inoculation solution without Magnaporthe conidia was used as a mock-inoculation control. Leaf samples were collected 24 hpi for transcriptomics analysis from three independent biological experiments. Leaf tissue was ground under liquid nitrogen and total RNA extracted using a QIAquick RNeasy Plant Extraction Kit (Qiagen, Hilden, Germany), followed by TURBO DNaseTM (Ambion, Texas, U.S.A.) treatment. RNeasy Mini Spin column purification (Qiagen) was used to further purify RNA samples for array hybridisation. RNA quality checks, cRNA conversion and Affymetrix genome array hybridisation was carried out by the Nottingham Arabidopsis Stock Centre (NASC) array hybridisation service (http://affymetrix.arabidopsis.info/).

Submitted at NCBI GEO via PLEXdb.

Publication: 'Wheat blast: histopathology and transcriptome reprogramming in response to adapted and nonadapted Magnaporthe isolates', Hale A. Tufan, Graham R. D. McGrann, Andreas Magusin, Jean-Benoit Morel, Lucie Mich, Lesley A. Boyd
New Phytologist Volume 184(2):473-484 (2009)
Created: 2008-12-19 01:43:59
Last Update: 2009-01-07 09:17:42
Released: 2009-09-28
GEO Accession GSE31760
Submitter: Graham Robert David McGrann
Name: Graham McGrann
Institution: John Innes Centre
Head of Laboratory: Lesley Boyd
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