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Browse WheatPLEX Experiment Data

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Expression data from rust or mock inoculated, fully expanded flag leaf halves
Jianfa,,Bai, Kansas State University, Manhattan, KS (jianfa@ksu.edu)
Experiment design (36 hybridizations)
genotype
•Jupateco_Lr34 (resistant) •Jupateco_Lr34(susceptible) •Thatcher_Lr34(resistant) •Thatcher_L34(susceptible)
organism part name
•basal leaf •distal leaf
treatment
•mock_inoculated •rust_inoculated

This experiment has been imported by PLEXdb from NCBI GEO (GSE6227)

Series_summary: Two sets of wheat lines near-isogenic to Lr34 were u...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Expression data from rust or mock inoculated, fully expanded flag leaf halves
Accession No: TA29
Microarray: Wheat
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•Jupateco_Lr34 (resistant)   •Jupateco_Lr34(susceptible)   •Thatcher_Lr34(resistant)   •Thatcher_L34(susceptible)
organism part name
•basal leaf   •distal leaf
treatment
•mock_inoculated   •rust_inoculated
Quality Control: biological replicates
Treatment summary:
 genotype  organism part name  treatment  # replicates
 Jupateco_Lr34 (resistant)  basal leaf  mock_inoculated  3
 Jupateco_Lr34 (resistant)  basal leaf  rust_inoculated  0
 Jupateco_Lr34 (resistant)  distal leaf  mock_inoculated  3
 Jupateco_Lr34 (resistant)  distal leaf  rust_inoculated  0
 Jupateco_Lr34(susceptible)  basal leaf  mock_inoculated  3
 Jupateco_Lr34(susceptible)  basal leaf  rust_inoculated  0
 Jupateco_Lr34(susceptible)  distal leaf  mock_inoculated  3
 Jupateco_Lr34(susceptible)  distal leaf  rust_inoculated  0
 Thatcher_Lr34(resistant)  basal leaf  mock_inoculated  3
 Thatcher_Lr34(resistant)  basal leaf  rust_inoculated  3
 Thatcher_Lr34(resistant)  distal leaf  mock_inoculated  3
 Thatcher_Lr34(resistant)  distal leaf  rust_inoculated  3
 Thatcher_L34(susceptible)  basal leaf  mock_inoculated  3
 Thatcher_L34(susceptible)  basal leaf  rust_inoculated  3
 Thatcher_L34(susceptible)  distal leaf  mock_inoculated  3
 Thatcher_L34(susceptible)  distal leaf  rust_inoculated  3
Total hybridizations: 36
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE6227)

Series_summary:
Two sets of wheat lines near-isogenic to Lr34 were used to compare gene expression profiles of wheat: 1. with and without Lr34 gene; 2. rust and mock inoculation; 3. distal and basal portion of the flag leaves. The two sets of wheat near-isogenic lines were used to subtract genetic background variations and to enrich Lr34-regulated gene expression profiles. The study is aimed to better understand the mechanisms of the well-known durable leaf rust resistance gene, Lr34, mediated resistance at the transcriptome level.

Keywords: Distal and basal leaf halves of near-isogenic lines

Series_overall_design:
Wheat near-isogenic lines, Jupateco with Lr34 (JUR), Jupateco without Lr34 (JUS), Thatcher with Lr34 (THR), and Thatcher without Lr34 (THS) were used. Thatcher lines were rust (I) or mock (M) inoculated. Jupateco lines were mock inoculated. Distal (T) and basal (B) half of the leaves were harvested and processed separately. Three biological replications were applied to each treatment.
Publication: none
Created: 2009-07-21 15:59:05
Last Update: 2009-07-21 16:29:06
GEO Accession GSE6227
Submitter: PLEXdb Curator
Name: Jianfa,,Bai
Institution: Kansas State University, Manhattan, KS
Head of Laboratory: --
email(s):
Homepage:

 
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