Search   for 
Find Your Gene ·  Publications ·  Tools ·  Gene List Suite ·  Expression Atlases ·  About PLEXdb ·  Feedback
Login ID:
Password:
Plants
Arabidopsis
Barley
Brachypodium
Citrus
Cotton
Grape
Maize
Medicago
Poplar
Rice
Soybean
Sugarcane
Tomato
Wheat
Pathogens & Symbionts
Search Experiments
Download Data
Gene List Suite
Tools
Your Account
Documentation
News
Personnel
Links
 Data Release Policy 
PLEXdb is committed to making expression data publicly available to researchers world-wide...
complete policy
 

Browse WheatPLEX Experiment Data

Choose an experiment:
Genotypic differences in water soluble carbohydrate metabolism in stem
Gang-Ping,,Xue, CSIRO, Brisbane, Australia (Gang-Ping.Xue@csiro.au)
Experiment design (16 hybridizations)
genotype
•SB165 •SB169 •SB10 •SB62 •SB109 •SB69 •SB57 •SB3

This experiment has been imported by PLEXdb from NCBI GEO (GSE9767)

Series_summary: Water soluble carbohydrates (WSC, composed of mainl...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

Show
Experiment Name: Genotypic differences in water soluble carbohydrate metabolism in stem
Accession No: TA30
Microarray: Wheat
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•SB165   •SB169   •SB10   •SB62   •SB109   •SB69   •SB57   •SB3
Quality Control: biological replicates
Treatment summary:
 genotype  # replicates
 SB165  2
 SB169  2
 SB10  2
 SB62  2
 SB109  2
 SB69  2
 SB57  2
 SB3  2
Total hybridizations: 16
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE9767)

Series_summary:
Water soluble carbohydrates (WSC, composed of mainly fructans, sucrose, glucose and fructose) deposited in wheat stems are important carbon sources for grain filling. Variation in stem WSC concentrations among wheat genotypes is one of the genetic factors influencing grain weight and yield under water-limited environments. Here, we describe the molecular dissection of carbohydrate metabolism in stems, at the WSC accumulation phase, of recombinant inbred SB (Seri/Babax) lines of Triticum aestivum differing in stem WSC concentrations. Affymetrix GeneChip analysis of carbohydrate metabolic enzymes revealed that the mRNA levels of two fructan synthetic enzyme families (sucrose:sucrose 1-fructosyltransferase and sucrose:fructan 6-fructosyltransferase) in the stem were positively correlated with stem WSC and fructan concentrations, while the mRNA levels of enzyme families involved in sucrose hydrolysis (sucrose synthase and soluble acid invertase) were inversely correlated with WSC concentrations. Differential regulation of the mRNA levels of these sucrose hydrolytic enzymes in SB lines resulted in genotypic differences in these enzyme activities. Down-regulation of sucrose synthase and soluble acid invertase in high WSC lines was accompanied by significant decreases in the mRNA levels of enzyme families related to sugar catabolic pathways (fructokinase and mitochondrion pyruvate dehydrogenase complex) and enzyme families involved in diverting UDP-glucose to cell wall synthesis (UDP-glucose 6-dehydrogenase, UDP-glucuronate decarboxylase and cellulose synthase), resulting in a reduction in cell wall polysaccharide contents (mainly hemicellulose) in the stem of high WSC lines. These data suggest that differential carbon partitioning in the wheat stem is one mechanism that contributes to genotypic variation in WSC accumulation.
We used Affymetrix GeneChip to dissect genotypic variation in carbohydrate metabolism related to water soluble carbohydrate accumulation in stems of wheat.

Keywords: Genotypic differences in water soluble carbohydrate metabolism in stem

Series_overall_design:
8 genotypes of recombinant inbred lines Seri M82 x Babax with 2 biological replicates per genotype. Grown in the field under rain-fed conditions
Publication: 'Molecular dissection of variation in carbohydrate metabolism related to water-soluble carbohydrate accumulation in stems of wheat', Xue GP, McIntyre CL, Jenkins CL, Glassop D et al
Plant Physiol 2008 Feb;146(2):441-54
pubmed: 18083795
Created: 2009-07-21 16:30:55
Last Update: 2009-07-21 16:47:24
GEO Accession GSE9767
Submitter: PLEXdb Curator
Name: Gang-Ping,,Xue
Institution: CSIRO, Brisbane, Australia
Head of Laboratory: --
email(s):
Homepage:

 
Copyright@2001-2013 The PLEXdb Group
All rights reserved.

For problems with the webpages contact PLEXdb webmasters