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Browse WheatPLEX Experiment Data

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Transcript profiling of Lr1- and Lr34-mediated leaf rust resistance in wheat
David,,Garvin, USDA, St. Paul, MN (David.Garvin@ars.usda.gov)
Experiment design (36 hybridizations)
genotype
•Tc •Tc-Lr1 •Tc-Lr34
time
•3 dpi •7 dpi
inoculation
•mock •P. triticina

This experiment has been imported by PLEXdb from NCBI GEO (GSE9915)

Series_summary: The wheat gene Lr34 confers partial resistance to a...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Transcript profiling of Lr1- and Lr34-mediated leaf rust resistance in wheat
Accession No: TA32
Microarray: Wheat
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•Tc   •Tc-Lr1   •Tc-Lr34
time
•3 dpi  •7 dpi
inoculation
•mock   •P. triticina
Quality Control: biological replicates
Treatment summary:
 genotype  time  inoculation  # replicates
 Tc  3 dpi  mock  3
 Tc  3 dpi  P. triticina  3
 Tc  7 dpi  mock  3
 Tc  7 dpi  P. triticina  3
 Tc-Lr1  3 dpi  mock  3
 Tc-Lr1  3 dpi  P. triticina  3
 Tc-Lr1  7 dpi  mock  3
 Tc-Lr1  7 dpi  P. triticina  3
 Tc-Lr34  3 dpi  mock  3
 Tc-Lr34  3 dpi  P. triticina  3
 Tc-Lr34  7 dpi  mock  3
 Tc-Lr34  7 dpi  P. triticina  3
Total hybridizations: 36
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE9915)

Series_summary:
The wheat gene Lr34 confers partial resistance to all races of Puccinia triticina, the causal agent of wheat leaf rust. However, the biological basis for the exceptional durability of Lr34 is unclear. The Affymetrix wheat genome array was used to identify wheat genes differentially expressed in a compatible interaction (Tc), an R-gene mediated incompatible interaction (Tc-Lr1), and a race non-specific resistance interaction (Tc-Lr34) in response to infection challenge by P. triticina race 1 at anthesis. Transcriptome interrogation was conducted by comparing mock- and P. triticina-inoculated leaves harvested at 3 and 7 days post inoculation (dpi).

Keywords: Time course


Series_overall_design:
The Affymetrix wheat genome array was used to identify wheat genes differentially expressed in a compatible interaction (Tc), an R-gene mediated incompatible interaction (Tc-Lr1), and a race non-specific resistance interaction (Tc-Lr34) in response to infection challenge by P. triticina race 1 at anthesis. Transcriptome interrogation was conducted on leaves harvested at 3 and 7 days post inoculation (dpi). The study utilized a randomized complete block design with three replicates for each genotype, and employed univariate analysis (t-tests) between mock- and P. triticina-inoculated plants within each genotype at each timepoint, for a total of six comparisons across the entire experiment, utilizing a total 36 Affymetrix Wheat Genome Array GeneChips.
Publication: 'Lr34-mediated leaf rust resistance in wheat: transcript profiling reveals a high energetic demand supported by transient recruitment of multiple metabolic pathways', Bolton MD, Kolmer JA, Xu WW, Garvin DF
Mol Plant Microbe Interact 2008 Dec;21(12):1515-27
pubmed: 18986248
Created: 2009-07-22 12:38:27
Last Update: 2009-07-22 13:15:31
GEO Accession GSE9915
Submitter: PLEXdb Curator
Name: David,,Garvin
Institution: USDA, St. Paul, MN
Head of Laboratory: --
email(s):
Homepage:

 
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