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RNA profiling of Fusarium head blight-resistant wheat addition lines containing the Thinopyrum elongatum chromosome 7E
Therese Ouellet, Agriculture and Agri-Food Canada, Department of Eastern Cereal and Oilseed Research Centre, Ottawa, Canada (therese.ouellet@agr.gc.ca)
Experiment design (18 hybridizations)
genotype
•CS •CS-7E •CS-7EL
pathogen inoculation
•FG •Control
time
•2 dpi •4 dpi

This experiment has been imported by PLEXdb from NCBI GEO (GSE21386)

Series_summary:
Fusarium head blight (FHB) is a major disease of...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: RNA profiling of Fusarium head blight-resistant wheat addition lines containing the Thinopyrum elongatum chromosome 7E
Accession No: TA35
Microarray: Wheat
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•CS   •CS-7E   •CS-7EL
pathogen inoculation
•FG   •Control
time
•2 dpi  •4 dpi
Quality Control: biological replicates
Treatment summary:
 genotype  pathogen inoculation  time  # replicates
 CS  FG  2 dpi  2
 CS  FG  4 dpi  3
 CS  Control  2 dpi  0
 CS  Control  4 dpi  2
 CS-7E  FG  2 dpi  2
 CS-7E  FG  4 dpi  3
 CS-7E  Control  2 dpi  0
 CS-7E  Control  4 dpi  0
 CS-7EL  FG  2 dpi  2
 CS-7EL  FG  4 dpi  2
 CS-7EL  Control  2 dpi  0
 CS-7EL  Control  4 dpi  2
Total hybridizations: 18
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE21386)

Series_summary:
Fusarium head blight (FHB) is a major disease of cereal crops caused by the fungus Fusarium graminearum (Fg). FHB affecting the flowering heads (or spikes). A FHB resistance locus has been identified on the chromosome 7E of the wild wheat relative Thinopyrum elongatum (Th.e.). That chromosome (7E) or a long arm fragment of it (7EL) have been transferred as additions in the wheat background 'Chinese Spring' (CS).
The two addition lines are resistant to FHB while 'Chinese Spring' is moderately susceptible to it. The mechanism of resistance is not known. The analysis of this work is published in the Canadian Journal of Plant Pathology (Wang et al, 2010).

Series_overall_design:
We used the wheat microarray to determine the global expression profil in inoculated spikelets of the addition and parental lines, after water or Fg treatment, with samplings at 2 and 4 days after inoculation (DAI).
Publication: 'RNA profiling of fusarium head blight-resistant wheat addition lines containing the Thinopyrum elongatum chromosome 7E.', Wang, J. -R., Wang, L., Gulden, S., Rocheleau, H., Balcerzak, M., Hattori, J., Cao, W., Han, F., Zheng, Y. -L., Fedak, G. and Ouellet, T.
Canadian Journal of Plant Pathology, Volume 32, Issue 2 April 2010, pages 188 - 214. DOI: 10.1080/07060661003740512
Created: 2011-05-10 12:35:35
Last Update: 2011-06-14 18:19:03
Released: 2011-06-14
GEO Accession GSE21386
Submitter: PLEXdb Curator
Name: Therese Ouellet
Institution: Agriculture and Agri-Food Canada, Department of Eastern Cereal and Oilseed Research Centre, Ottawa, Canada
Head of Laboratory: Therese Ouellet
email(s):
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