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Browse WheatPLEX Experiment Data

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Metabolism-based resistance to herbicides in black-grass
Sandrine Balzergue, INRA, Department of URGV, Evry, France (balzerg@evry.inra.fr)
Experiment design (6 hybridizations)
genotype
•Sensitive •Moderately resistant •Resistant

This experiment has been imported by PLEXdb from NCBI GEO (GSE15864)

Series_summary:
Resistance to herbicides in weeds can be due to ...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Metabolism-based resistance to herbicides in black-grass
Accession No: TA37
Microarray: Wheat
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•Sensitive   •Moderately resistant   •Resistant
Quality Control: biological replicates
Treatment summary:
 genotype  # replicates
 Sensitive  2
 Moderately resistant  2
 Resistant  2
Total hybridizations: 6
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE15864)

Series_summary:
Resistance to herbicides in weeds can be due to alteration(s) in the gene encoding the herbicide target site, or to herbicide degradation via a deviation in plant general metabolism. If target-site-based resistance is easy to study, the multigenic control of metabolism-based resistance renders it much more complex to study. Metabolism-based resistance to herbicides represents the major part of herbicide resistance in black-grass. Its most likely basis is an overexpression of genes encoding enzymes degrading herbicides. We thus seek to identify such overexpressed genes by comparing the transcriptomes of resistant and sensitive black-grass individuals belonging to an F2 line segregating for two resistance genes. Given there are no genomic tools developed for black-grass, this approach will use heterologous hybridisation onto a wheat Affymetrix microarray.

Comparison using heterologous hybridisation onto a wheat whole-genome microarray of transcriptome of three pools of black-grass plants obtained 2h30 after herbicide spraying at field rate. The three pools correspond to: · Sensitive plants (killed by herbicide). · Moderately resistant plants (growth impaired by herbicide but plants still alive) · Resistant plants (growth unimpaired by herbicide)

Series_overall_design:
6 arrays - wheat

Curator's Comment:
Please note that the correlation between replicates within a treatment is low in case of MAS normalized data, but the RMA data doesn't show this low correlation. This can be viewed from the Quality Control tab.
Publication: none
Created: 2011-05-16 14:23:55
Last Update: 2011-06-17 14:38:40
Released: 2011-06-17
GEO Accession GSE15864
Submitter: PLEXdb Curator
Name: Sandrine Balzergue
Institution: INRA, Department of URGV, Evry, France
Head of Laboratory: --
email(s):
Homepage:

 
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