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Expression data in wheat (T. aestivum L.) near isogenic lines in response to powdery mildew infection
Mingming Xin, China Agricultrual University, Department of Plant Genetics and Breeding, Beijing, China (xmmlyac@126.com)
Experiment design (12 hybridizations)
genotype
•susceptible(8866) •resistant(pm30)
time
•0 hrs •12 hrs

This experiment has been imported by PLEXdb from NCBI GEO (GSE27320)

Series_summary:
We used two wheat genotypes, the susceptible whe...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Expression data in wheat (T. aestivum L.) near isogenic lines in response to powdery mildew infection
Accession No: TA39
Microarray: Wheat
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•susceptible(8866)   •resistant(pm30)
time
•0 hrs  •12 hrs
Quality Control: biological replicates
Treatment summary:
 genotype  time  # replicates
 susceptible(8866)  0 hrs  3
 susceptible(8866)  12 hrs  3
 resistant(pm30)  0 hrs  3
 resistant(pm30)  12 hrs  3
Total hybridizations: 12
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE27320)

Series_summary:
We used two wheat genotypes, the susceptible wheat cultivar ‘8866 ’(S) and its near isogenic line with single powdery mildew resistance gene ‘pm30’ (R), to investigate gene expression changes in response to powdery mildew infection by using Wheat Genome Array

Series_overall_design:
wheat young leveas of near isogenic lines before or 12 hours after powdery mildew infection were selected for RNA extraction and hybridization on Affymetrix microarrays.The leaf samples were harvested from three independent biological replicates, and the leaves without inoculation were regarded as control.

PLEXdb Curator's Comment:

At GEO this sub-series is part of SuperSeries, GSE27339 (Wheat response to powdery mildew infection and heat stress) which has not been imported into PLEXdb because it contains samples from 2 different platforms.
Publication: 'Identification and characterization of wheat long non-protein coding RNAs responsive to powdery mildew infection and heat stress by using microarray analysis and SBS sequencing.', Mingming Xin, Yu Wang, Yingyin Yao, Na Song, Zhaorong Hu, Dandan Qin, Chaojie Xie, Huiru Peng, Zhongfu Ni,and Qixin Sun.
BMC Plant Biol 2011 Apr 7;11:61.
pubmed: 21473757
Created: 2011-08-12 10:20:55
Last Update: 2011-08-16 12:35:09
Released: 2011-08-16
GEO Accession GSE27320
Submitter: PLEXdb Curator
Name: Mingming Xin
Institution: China Agricultrual University, Department of Plant Genetics and Breeding, Beijing, China
Head of Laboratory: Qixin Sun
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