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Differential transcriptome analyses of three wheat genotypes in their response to Fusarium Head Blight and trichothecenes
Nora Foroud, Agriculture and Agri-Foods Canada, Lethbridge, Canada (nora.foroud@agr.gc.ca)
Experiment design (144 hybridizations)
genotype
•Superb •GS-1-EM0040 •GS-1-EM0168
pathogen inoculation
•uninoculated control •water •FgTri5+ •FgTri5- •FgTri5-/DON •DON
time
•0 hrs •3 hrs •8 hrs •24 hrs

This experiment has been imported by PLEXdb from NCBI GEO (GSE28973)

Series_summary:
Fusarium Head Blight (FHB) is a disease of wheat...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Differential transcriptome analyses of three wheat genotypes in their response to Fusarium Head Blight and trichothecenes
Accession No: TA40
Microarray: Wheat
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•Superb   •GS-1-EM0040   •GS-1-EM0168
pathogen inoculation
•uninoculated control   •water   •FgTri5+   •FgTri5-   •FgTri5-/DON   •DON
time
•0 hrs  •3 hrs  •8 hrs  •24 hrs
Quality Control: biological replicates
Treatment summary:
 genotype  pathogen inoculation  time  # replicates
 Superb  uninoculated control  0 hrs  3
 Superb  uninoculated control  3 hrs  0
 Superb  uninoculated control  8 hrs  0
 Superb  uninoculated control  24 hrs  0
 Superb  water  0 hrs  0
 Superb  water  3 hrs  3
 Superb  water  8 hrs  3
 Superb  water  24 hrs  3
 Superb  FgTri5+  0 hrs  0
 Superb  FgTri5+  3 hrs  3
 Superb  FgTri5+  8 hrs  3
 Superb  FgTri5+  24 hrs  3
 Superb  FgTri5-  0 hrs  0
 Superb  FgTri5-  3 hrs  3
 Superb  FgTri5-  8 hrs  3
 Superb  FgTri5-  24 hrs  3
 Superb  FgTri5-/DON  0 hrs  0
 Superb  FgTri5-/DON  3 hrs  3
 Superb  FgTri5-/DON  8 hrs  3
 Superb  FgTri5-/DON  24 hrs  3
 Superb  DON  0 hrs  0
 Superb  DON  3 hrs  3
 Superb  DON  8 hrs  3
 Superb  DON  24 hrs  3
 GS-1-EM0040  uninoculated control  0 hrs  3
 GS-1-EM0040  uninoculated control  3 hrs  0
 GS-1-EM0040  uninoculated control  8 hrs  0
 GS-1-EM0040  uninoculated control  24 hrs  0
 GS-1-EM0040  water  0 hrs  0
 GS-1-EM0040  water  3 hrs  3
 GS-1-EM0040  water  8 hrs  3
 GS-1-EM0040  water  24 hrs  3
 GS-1-EM0040  FgTri5+  0 hrs  0
 GS-1-EM0040  FgTri5+  3 hrs  3
 GS-1-EM0040  FgTri5+  8 hrs  3
 GS-1-EM0040  FgTri5+  24 hrs  3
 GS-1-EM0040  FgTri5-  0 hrs  0
 GS-1-EM0040  FgTri5-  3 hrs  3
 GS-1-EM0040  FgTri5-  8 hrs  3
 GS-1-EM0040  FgTri5-  24 hrs  3
 GS-1-EM0040  FgTri5-/DON  0 hrs  0
 GS-1-EM0040  FgTri5-/DON  3 hrs  3
 GS-1-EM0040  FgTri5-/DON  8 hrs  3
 GS-1-EM0040  FgTri5-/DON  24 hrs  3
 GS-1-EM0040  DON  0 hrs  0
 GS-1-EM0040  DON  3 hrs  3
 GS-1-EM0040  DON  8 hrs  3
 GS-1-EM0040  DON  24 hrs  3
 GS-1-EM0168  uninoculated control  0 hrs  3
 GS-1-EM0168  uninoculated control  3 hrs  0
 GS-1-EM0168  uninoculated control  8 hrs  0
 GS-1-EM0168  uninoculated control  24 hrs  0
 GS-1-EM0168  water  0 hrs  0
 GS-1-EM0168  water  3 hrs  3
 GS-1-EM0168  water  8 hrs  3
 GS-1-EM0168  water  24 hrs  3
 GS-1-EM0168  FgTri5+  0 hrs  0
 GS-1-EM0168  FgTri5+  3 hrs  3
 GS-1-EM0168  FgTri5+  8 hrs  3
 GS-1-EM0168  FgTri5+  24 hrs  3
 GS-1-EM0168  FgTri5-  0 hrs  0
 GS-1-EM0168  FgTri5-  3 hrs  3
 GS-1-EM0168  FgTri5-  8 hrs  3
 GS-1-EM0168  FgTri5-  24 hrs  3
 GS-1-EM0168  FgTri5-/DON  0 hrs  0
 GS-1-EM0168  FgTri5-/DON  3 hrs  3
 GS-1-EM0168  FgTri5-/DON  8 hrs  3
 GS-1-EM0168  FgTri5-/DON  24 hrs  3
 GS-1-EM0168  DON  0 hrs  0
 GS-1-EM0168  DON  3 hrs  3
 GS-1-EM0168  DON  8 hrs  3
 GS-1-EM0168  DON  24 hrs  3
Total hybridizations: 144
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE28973)

Series_summary:
Fusarium Head Blight (FHB) is a disease of wheat and other cereal crops, where Fusarium graminearum and related species infects the wheat inflorescence during and post-anthesis. The fungus produces trichothecene toxins that accumulate in the grain of infected head, and are required for disease spread. Microarrays were used to observe differential gene expression in the uninoculated spikelets of FHB-challenged wheat spikes in three wheat genotypes. A summary of our findings will be published in Plant Pathology.

Series_overall_design:
Three wheat genotypes were used: (1) 'Superb', an FHB-susceptible Canadian wheat cultivar; (2) GS-1-EM0040 (CIMMYT11x'Superb'*2), a double haploid line with good resistance to initial infection (Type 1 resistance), and moderate resistance to disease spread (Type 2 resistance); and (3) GS-1-EM0168 (CM82036x'Superb'*2), a double haploid line with moderate Type 1 resistance, and good Type 2 resistance. Five inocula were used: (A) water, (B) FgTri5+ (GZ3639, a trichothecene-producing F. graminearum strain); (C) FgTri5- (GZT40, a trichothecene-non-producing mutant of the F. graminearum strain GZ3639); (D) FgTri5 supplemented with deoxynivalenol (DON), which is the main trichothecene produced by FgTri5+; and (E) DON. The inocula were injected into two spikelets near the center of the spike during early stages of anthesis, and spikelets above and below the inoculation point were collected at 3, 8, and 24 h after inoculation. A zero-hour un-inoculated control was also collected from each line. Total RNA was extracted from collected spikelets, and microarray analysis was perfomed using the Affymetrix Wheat GeneChip.
Publication: none
Created: 2011-10-20 15:58:28
Last Update: 2011-10-27 20:53:50
Released: 2011-10-27
GEO Accession GSE28973
Submitter: PLEXdb Curator
Name: Nora Foroud
Institution: Agriculture and Agri-Foods Canada, Lethbridge, Canada
Head of Laboratory: Brian Ellis
email(s):
Homepage:

 
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