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Response to Fusarium in near-isogenic lines harboring resistance QTLs Fhb1 and Qfhs.ifa-5A
Wolfgang Schweiger, Institute for Biotechnology in Plant Production,University of Natural Resources and Life Sciences, Vienna (wolfgang.schweiger@boku.ac.at)
Experiment design (90 hybridizations)
genotype
•NIL1 (resistant, Fhb1, Qfhs.ifa-5A) •NIL2 (moderately resistant, Fhb1) •NIL3 (moderately resistant, Qfhs.ifa-5A) •NIL4 (susceptible) •CM82036 (resistant)
treatment
•Fusarium graminearum strain IFA-65 •water
time
•8 hai •24 hai •72 hai

Resistance mechanisms of wheat to Fusarium graminearum are still poorly understood. Multiple reports have investigated transcriptomic differences b...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Response to Fusarium in near-isogenic lines harboring resistance QTLs Fhb1 and Qfhs.ifa-5A
Accession No: TA41
Microarray: Wheat
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•NIL1 (resistant, Fhb1, Qfhs.ifa-5A)   •NIL2 (moderately resistant, Fhb1)   •NIL3 (moderately resistant, Qfhs.ifa-5A)   •NIL4 (susceptible)   •CM82036 (resistant)
treatment
•Fusarium graminearum strain IFA-65   •water
time
•8 hai  •24 hai  •72 hai
Quality Control: biological replicates
Treatment summary:
 genotype  treatment  time  # replicates
 NIL1 (resistant, Fhb1, Qfhs.ifa-5A)  Fusarium graminearum strain IFA-65  8 hai  3
 NIL1 (resistant, Fhb1, Qfhs.ifa-5A)  Fusarium graminearum strain IFA-65  24 hai  3
 NIL1 (resistant, Fhb1, Qfhs.ifa-5A)  Fusarium graminearum strain IFA-65  72 hai  3
 NIL1 (resistant, Fhb1, Qfhs.ifa-5A)  water  8 hai  3
 NIL1 (resistant, Fhb1, Qfhs.ifa-5A)  water  24 hai  3
 NIL1 (resistant, Fhb1, Qfhs.ifa-5A)  water  72 hai  3
 NIL2 (moderately resistant, Fhb1)  Fusarium graminearum strain IFA-65  8 hai  3
 NIL2 (moderately resistant, Fhb1)  Fusarium graminearum strain IFA-65  24 hai  3
 NIL2 (moderately resistant, Fhb1)  Fusarium graminearum strain IFA-65  72 hai  3
 NIL2 (moderately resistant, Fhb1)  water  8 hai  3
 NIL2 (moderately resistant, Fhb1)  water  24 hai  3
 NIL2 (moderately resistant, Fhb1)  water  72 hai  3
 NIL3 (moderately resistant, Qfhs.ifa-5A)  Fusarium graminearum strain IFA-65  8 hai  3
 NIL3 (moderately resistant, Qfhs.ifa-5A)  Fusarium graminearum strain IFA-65  24 hai  3
 NIL3 (moderately resistant, Qfhs.ifa-5A)  Fusarium graminearum strain IFA-65  72 hai  3
 NIL3 (moderately resistant, Qfhs.ifa-5A)  water  8 hai  3
 NIL3 (moderately resistant, Qfhs.ifa-5A)  water  24 hai  3
 NIL3 (moderately resistant, Qfhs.ifa-5A)  water  72 hai  3
 NIL4 (susceptible)  Fusarium graminearum strain IFA-65  8 hai  3
 NIL4 (susceptible)  Fusarium graminearum strain IFA-65  24 hai  3
 NIL4 (susceptible)  Fusarium graminearum strain IFA-65  72 hai  3
 NIL4 (susceptible)  water  8 hai  3
 NIL4 (susceptible)  water  24 hai  3
 NIL4 (susceptible)  water  72 hai  3
 CM82036 (resistant)  Fusarium graminearum strain IFA-65  8 hai  3
 CM82036 (resistant)  Fusarium graminearum strain IFA-65  24 hai  3
 CM82036 (resistant)  Fusarium graminearum strain IFA-65  72 hai  3
 CM82036 (resistant)  water  8 hai  3
 CM82036 (resistant)  water  24 hai  3
 CM82036 (resistant)  water  72 hai  3
Total hybridizations: 90
Description: Resistance mechanisms of wheat to Fusarium graminearum are still poorly understood. Multiple reports have investigated transcriptomic differences between resistant and susceptible genotypes. To investigate the genetic determinants underlying the two major contributor QTL Fhb1 and Qfhs.ifa-5A, we generated a set of near-isogenic lines, that we have used for transcriptomic studies using the Affymetrix wheat GeneChip®:
4 near-isogenic lines (BC5F2) derived from a cross of CM82036 and cv Remus (susceptible recurrent parent) and the resistant parent CM82036 have been inoculated with Fusarium spore suspension or water as control at anthesis. Samples have been taken at 3 timepoints 8, 24 and 72 hours after inoculation. Five wheat heads per condition were pooled into one sample, that has been used for RNA preparation. Including 3 replicates a total of 90 microarrays have been hybridized.
Our results identify a wide variety of genes (631 transcripts) that are differentially regulated for Fusarium graminearum in all genotypes after 72 h and 339 genes that are upregulated only in lines without Qfhs.ifa.5A. Few genes were found differentially regulated for Fusarium and QTL. Many interesting candidates emerge from the set of QTL-specific genes that are constitutively expressed when comparing water-treated samples.

Submitted at NCBI GEO via PLEXdb.

Publication: none
Created: 2011-11-16 07:32:39
Last Update: 2011-11-17 17:04:29
Released: 2013-05-13
GEO Accession GSE43706
Submitter: Wolfgang Schweiger
Name: Wolfgang Schweiger
Institution: Institute for Biotechnology in Plant Production,University of Natural Resources and Life Sciences, Vienna
Head of Laboratory: Hermann Buerstmayr
email(s):
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