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Browse WheatPLEX Experiment Data

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Expression data from Triticum aestivum L. roots
Yongchun Li, Henan Agricultural University, Department of National Engineering Research Center for Wheat, Henan, China (yongchunli71@gmail.com)
Experiment design (4 hybridizations)
genotype
•Luohan •Chinese Spring
treatment
•control •PEG

This experiment has been imported by PLEXdb from NCBI GEO (GSE30872)

Series_summary:
To better understand the regulatory mechanisms o...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Expression data from Triticum aestivum L. roots
Accession No: TA43
Microarray: Wheat
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•Luohan   •Chinese Spring
treatment
•control   •PEG
Quality Control: biological replicates
Treatment summary:
 genotype  treatment  # replicates
 Luohan  control  1
 Luohan  PEG  1
 Chinese Spring  control  1
 Chinese Spring  PEG  1
Total hybridizations: 4
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE30872)

Series_summary:
To better understand the regulatory mechanisms of water stress response in wheat, the transcript profiles in roots of two wheat genotypes, namely, drought tolerant 'Luohan No.2' (LH) and drought susceptible 'Chinese Spring' (CS) under water-stress were comparatively analyzed by using the Affymetrix wheat GeneChip®.
A total of 3831 transcripts displayed 2-fold or more expression changes, 1593 transcripts were induced compared with 2238 transcripts were repressed, in LH under water-stress; Relatively fewer transcripts were drought responsive in CS, 1404 transcripts were induced and 1493 were repressed. Comparatively, 569 transcripts were commonly induced and 424 transcripts commonly repressed in LH and CS under water-stress. 689 transcripts (757 probe sets) identified from LH and 537 transcripts (575 probe sets) from CS were annotated and classified into 10 functional categories, and 74 transcripts derived from 80 probe sets displayed the change ratios no less than 16 in LH or CS. Several kinds of candidate genes were differentially expressed between the LH and CS, which could be responsible for the difference in drought tolerance of the two genotypes.

Series_overall_design:
Two common wheat (Triticum aestivum L.) cultivars, Luohan No.2 (LH) and Chinese Spring (CS), were used for this study. Seedlings at the two leaf stage were stressed by cultured in PEG solutions for 6h, and some other seedlings were cultured in tap water as control. Root samples of LH and CS at 6h after the stress treatment and untreated control were prepared for microarray analysis.
Publication: none
Created: 2012-03-27 11:28:52
Last Update: 2012-03-27 13:11:08
Released: 2012-03-29
GEO Accession GSE30872
Submitter: PLEXdb Curator
Name: Yongchun Li
Institution: Henan Agricultural University, Department of National Engineering Research Center for Wheat, Henan, China
Head of Laboratory: --
email(s):
Homepage:

 
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