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Browse WheatPLEX Experiment Data

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Transcriptome profiling of reproductive stage flag leaves of wheat from drought susceptible parent WL711, drought tolerant parent C306 and drought susceptible and drought tolerant RIL bulks in irrigated and drought condition
Suhas Baliram Kadam, NRCPb, Department of Biotechnology, New Dehli, India (suhaskad@gmail.com)
Experiment design (24 hybridizations)
cultivar
•WL711 susceptible •RIL susceptible •C306 tolerant •RIL tolerant
stress condition
•irrigated •drought

This experiment has been imported by PLEXdb from NCBI GEO (GSE30436)

Series_summary:
The aim of the study was to identify candidate g...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Transcriptome profiling of reproductive stage flag leaves of wheat from drought susceptible parent WL711, drought tolerant parent C306 and drought susceptible and drought tolerant RIL bulks in irrigated and drought condition
Accession No: TA44
Microarray: Wheat
Visibility: public
Experiment Type:
Experiment Factor(s):
cultivar
•WL711 susceptible   •RIL susceptible   •C306 tolerant   •RIL tolerant
stress condition
•irrigated   •drought
Quality Control: biological replicates
Treatment summary:
 cultivar  stress condition  # replicates
 WL711 susceptible  irrigated  3
 WL711 susceptible  drought  3
 RIL susceptible  irrigated  3
 RIL susceptible  drought  3
 C306 tolerant  irrigated  3
 C306 tolerant  drought  3
 RIL tolerant  irrigated  3
 RIL tolerant  drought  3
Total hybridizations: 24
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE30436)

Series_summary:
The aim of the study was to identify candidate genes responsible for drought tolerance trait between a pair of wheat varieties ( WL711 and C306) and correspondng progeny bulks (10 drought susceptible RILs and 10 drought tolerant RILs) by combining QTLs analysis with expression analysis. Microarray analysis of RNA extracted from the flag leaves showed large number of differentially expressed genes. The number of differentially expressed genes was reduced to 37 on the basis of their occurance in a major QTL region (responcible for drought tolerance) detected in RIL population derived from WL711 and C306.

Series_overall_design:
RNA from the reproductive stage flag leaf of wheat in irrigated and drought condition of drought susceptible parent WL711, drought tolerant parent C306 and drought susceptible and drought tolerant RIL bulks were analysed in three different biological replications (A , B and C) making total twenty four samples
Publication: 'Genomic associations for drought tolerance on the short arm of wheat chromosome 4B.', Kadam S, Singh K, Shukla S, Goel S, Vikram P, Pawar V, Gaikwad K, Khanna-Chopra R, Singh N.
Funct Integr Genomics 2012 Apr 5.
pubmed: 22476619
Created: 2012-05-17 12:01:47
Last Update: 2012-05-17 15:47:00
Released: 2012-05-23
GEO Accession GSE30436
Submitter: PLEXdb Curator
Name: Suhas Baliram Kadam
Institution: NRCPb, Department of Biotechnology, New Dehli, India
Head of Laboratory: NK Singh
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