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Global transgenerational gene expression dynamics in two nascent allohexaploid wheat lines analogous in genome constitution to common wheat (Triticum aestivum)
Bao Qi, Chinese Academy of Science, Changchun, China (geneprotein2003@yahoo.com.cn)
Experiment design (18 hybridizations)
genetic line
•AT5 selfed generation 4 •AT5 selfed generation 5 •AT9 selfed generation 4 •AT9 selfed generation 5 •MPV5 •MPV9 •Parental TQ27 •Parental TTH01 •Parental TTR04

This experiment has been imported by PLEXdb from NCBI GEO (GSE29882)

Series_summary:
Alteration in gene expression accompanying initi...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Global transgenerational gene expression dynamics in two nascent allohexaploid wheat lines analogous in genome constitution to common wheat (Triticum aestivum)
Accession No: TA45
Microarray: Wheat
Visibility: public
Experiment Type:
Experiment Factor(s):
genetic line
•AT5 selfed generation 4   •AT5 selfed generation 5   •AT9 selfed generation 4   •AT9 selfed generation 5   •MPV5   •MPV9   •Parental TQ27   •Parental TTH01   •Parental TTR04
Quality Control: biological replicates
Treatment summary:
 genetic line  # replicates
 AT5 selfed generation 4  2
 AT5 selfed generation 5  2
 AT9 selfed generation 4  2
 AT9 selfed generation 5  2
 MPV5  2
 MPV9  2
 Parental TQ27  2
 Parental TTH01  2
 Parental TTR04  2
Total hybridizations: 18
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE29882)

Series_summary:
Alteration in gene expression accompanying initial stages of allopolyploidy is a prominent feature in plants, but its spectrum and model are highly idiosyncratic. We used multi-colour GISH to identify individuals from two nascent allohexaploid wheat lines between Triticum turgidum and Aegilops tauschii, which had a transgenerationally stable chromosomal constitution mimicking that of common wheat. We performed genomewide analysis of gene expression for these plants along with their parental species using the Affymetrix GeneChip Wheat Genome-Array. Comparison with parental species coupled with inclusion of empirical mid-parent values (MPVs) revealed two patterns of alteration in gene expression in the allohexaploid lines: parental dominance expression and nonadditive expression. Genes involved in each altered pattern could be classified into three distinct groups, stochastic, heritable and persistent, based on their transgenerational heritability and inter-line conservation. Whereas both altered patterns of gene expression showed a propensity of inheritance, identity of the involved genes is stochastic, consistent with the involvement of diverse Gene Ontology (GO) terms. Nonetheless, those genes showing nonadditive expression exhibited a significant enrichment for vesicle-function. Our results suggest global alteration in gene expression conditioned by nascent allopolyploidy likely play functional roles in stabilization and establishment of the newly formed plants, and consequential to evolution.

Series_overall_design:
We used Affymetrix GeneChip Wheat Genome-Array to compare the expression difference of newly synthetic wheat allopolyploid lines and their parental lines.
Publication: none
Created: 2012-07-03 10:25:43
Last Update: 2012-07-03 13:50:02
Released: 2012-07-10
GEO Accession GSE29882
Submitter: PLEXdb Curator
Name: Bao Qi
Institution: Chinese Academy of Science, Changchun, China
Head of Laboratory: --
email(s):
Homepage:

 
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