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Browse WheatPLEX Experiment Data

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Alteration in transcript level in wheat alloplasmic lines
Cristina Crosatti, CRA-Genomics Research Centre (cristina.crosatti@entecra.it)
Experiment design (15 hybridizations)
genotype
•EU_T20 •ALLO_TH237 •EU_CHRIS •ALLO_183 •ALLO_195

Alloplasmic lines provide a unique tool to study the nucleo-cytoplasmic interactions. Alloplasmic lines T183 and T195 were developed through the in...[complete overview]

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Experiment Name: Alteration in transcript level in wheat alloplasmic lines
Accession No: TA49
Microarray: Wheat
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•EU_T20   •ALLO_TH237   •EU_CHRIS   •ALLO_183   •ALLO_195
Quality Control: biological replicates
Treatment summary:
 genotype  # replicates
 EU_T20  3
 ALLO_TH237  3
 EU_CHRIS  3
 ALLO_183  3
 ALLO_195  3
Total hybridizations: 15
Description: Alloplasmic lines provide a unique tool to study the nucleo-cytoplasmic interactions. Alloplasmic lines T183 and T195 were developed through the introgression of the cytoplasm from Aegilops uniaristata (T183) and Aegilops squarrosa (T195) in the nuclear background of Triticum aestivum cv. Chris. Alloplasmic line TH237 was produced introgressing the Hordeum chilense accession H7 cytoplasm into the nuclear background of Triticum aestivum accession T20. Fifty seeds for each sample in pots of 11 cm diameter and grown in controlled conditions under 600µE m-2 s–1 high light intensity in a daily regime of 12 h light at 22°C and 12 h darkness at 15°C. Plants were bulked from each pot and three biological replicate used for the transcriptomics Fully expanded second leaves were collected two weeks from sowing in the middle of the light period and used for transcriptomic analysis.

Submitted at NCBI GEO via PLEXdb.

Publication: none
Created: 2013-06-20 04:39:12
Last Update: 2014-01-02 11:58:01
Released: 2014-01-02
GEO Accession GSE48439
Submitter: cristina crosatti
Name: Cristina Crosatti
Institution: CRA-Genomics Research Centre
Head of Laboratory: Luigi Cattivelli
email(s):
Homepage:

 
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