Search   for 
Find Your Gene ·  Publications ·  Tools ·  Gene List Suite ·  Expression Atlases ·  About PLEXdb ·  Feedback
Login ID:
Password:
Plants
Arabidopsis
Barley
Brachypodium
Citrus
Cotton
Grape
Maize
Medicago
Poplar
Rice
Soybean
Sugarcane
Tomato
Wheat
Pathogens & Symbionts
Search Experiments
Download Data
Gene List Suite
Tools
Your Account
Documentation
News
Personnel
Links
 Data Release Policy 
PLEXdb is committed to making expression data publicly available to researchers world-wide...
complete policy
 

Browse GrapePLEX Experiment Data

Choose an experiment:
Photoperiod regulation of grape bud dormancy
Anne Fennell, South Dakota State University (anne.fennell@sdstate.edu)
Experiment design (84 hybridizations)
genotype
•Seyval (SV, Seyve Villard 5-276) •V. riparia (VR, PI588259)
other
•Long photoperiod (LD, 15h) •Short photoperiod (SD, 13h)
time
•1 days •3 days •7 days •14 days •21 days •28 days •42 days

Bud endodormancy induction response of two genotypes (‘Seyval’ a hybrid white wine grape and V. riparia, PI588259 a native north american speci...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

Show
Experiment Name: Photoperiod regulation of grape bud dormancy
Accession No: VV10
Microarray: Grape
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•Seyval (SV, Seyve Villard 5-276)   •V. riparia (VR, PI588259)
other
•Long photoperiod (LD, 15h)   •Short photoperiod (SD, 13h)
time
•1 days  •3 days  •7 days  •14 days  •21 days  •28 days  •42 days
Quality Control: biological replicates
Treatment summary:
 genotype  other  time  # replicates
 Seyval (SV, Seyve Villard 5-276)  Long photoperiod (LD, 15h)  1 days  3
 Seyval (SV, Seyve Villard 5-276)  Long photoperiod (LD, 15h)  3 days  3
 Seyval (SV, Seyve Villard 5-276)  Long photoperiod (LD, 15h)  7 days  3
 Seyval (SV, Seyve Villard 5-276)  Long photoperiod (LD, 15h)  14 days  3
 Seyval (SV, Seyve Villard 5-276)  Long photoperiod (LD, 15h)  21 days  3
 Seyval (SV, Seyve Villard 5-276)  Long photoperiod (LD, 15h)  28 days  3
 Seyval (SV, Seyve Villard 5-276)  Long photoperiod (LD, 15h)  42 days  3
 Seyval (SV, Seyve Villard 5-276)  Short photoperiod (SD, 13h)  1 days  3
 Seyval (SV, Seyve Villard 5-276)  Short photoperiod (SD, 13h)  3 days  3
 Seyval (SV, Seyve Villard 5-276)  Short photoperiod (SD, 13h)  7 days  3
 Seyval (SV, Seyve Villard 5-276)  Short photoperiod (SD, 13h)  14 days  3
 Seyval (SV, Seyve Villard 5-276)  Short photoperiod (SD, 13h)  21 days  3
 Seyval (SV, Seyve Villard 5-276)  Short photoperiod (SD, 13h)  28 days  3
 Seyval (SV, Seyve Villard 5-276)  Short photoperiod (SD, 13h)  42 days  3
 V. riparia (VR, PI588259)  Long photoperiod (LD, 15h)  1 days  3
 V. riparia (VR, PI588259)  Long photoperiod (LD, 15h)  3 days  3
 V. riparia (VR, PI588259)  Long photoperiod (LD, 15h)  7 days  3
 V. riparia (VR, PI588259)  Long photoperiod (LD, 15h)  14 days  3
 V. riparia (VR, PI588259)  Long photoperiod (LD, 15h)  21 days  3
 V. riparia (VR, PI588259)  Long photoperiod (LD, 15h)  28 days  3
 V. riparia (VR, PI588259)  Long photoperiod (LD, 15h)  42 days  3
 V. riparia (VR, PI588259)  Short photoperiod (SD, 13h)  1 days  3
 V. riparia (VR, PI588259)  Short photoperiod (SD, 13h)  3 days  3
 V. riparia (VR, PI588259)  Short photoperiod (SD, 13h)  7 days  3
 V. riparia (VR, PI588259)  Short photoperiod (SD, 13h)  14 days  3
 V. riparia (VR, PI588259)  Short photoperiod (SD, 13h)  21 days  3
 V. riparia (VR, PI588259)  Short photoperiod (SD, 13h)  28 days  3
 V. riparia (VR, PI588259)  Short photoperiod (SD, 13h)  42 days  3
Total hybridizations: 84
Description: Bud endodormancy induction response of two genotypes (‘Seyval’ a hybrid white wine grape and V. riparia, PI588259 a native north american species) was compared under long (15h) and short (13h) photoperiod. Three separate replicates (5 plants/replicate) were treated to generate paradormant (LD) and same aged endodormancy-induced (SD) buds for transcriptomic, proteomic and metabolomic analysis. Potted, spur-pruned two to six-year-old vines were removed from cold storage (Seyval 3-19-07; V. riparia 3/26/07) and grown under a LD (15 h) at 25/20 + 3C day/night temperatures (D/N). When vines reached 12-15 nodes (3-25-07) they were randomized into LD or SD treatments with 25/20 + 3C D/N in climate controlled greenhouses with automated photoperiod system (VRE Greenhouse Systems). Three replications (5 vines/replication) were harvested between 5/07-6/07 and then again in 5/08-6/08 for a total of six replications. All treatments are repeated at the same time every year and harvested at the same time of day each year to minimize biological noise. At 1, 3, 7, 14, 21, 28 and 42 days of LD and SD treatment, buds were harvested from nodes 3 to 12 of each separate replicate, immediately frozen in liquid nitrogen, and placed at -80C for future RNA, protein and metabolite extraction. These time points encompass early reversible phases as well as key time points during transition to irreversible endodormancy development. After photoperiod treatments and bud harvests, all pruned vines were returned to LD and monitored for bud endodormancy. The endodormant vines were identified after 28 days and moved to cold storage. The nondormant vines were allowed to grow again and induced into dormancy at a later date.
Acknowledgement:This study was funded by NSF Grant DBI0604755 and funds from the South Dakota Agriculture Experiment Station.

Submitted at NCBI GEO via PLEXdb
Publication: none
Created: 2007-10-05 16:32:12
Last Update: 2008-04-22 16:29:29
GEO Accession GSE17502
Submitter: Anne Y Fennell
Name: Anne Fennell
Institution: South Dakota State University
Head of Laboratory: Fennell
email(s):
Homepage:

 
Copyright@2001-2013 The PLEXdb Group
All rights reserved.

For problems with the webpages contact PLEXdb webmasters